瑞舒伐他汀对Medtronic球囊导管损伤大鼠颈动脉平滑肌细胞增殖及凋亡的影响  

Influence of Rosuvastatin on carotid artery smooth muscle cells proliferation and apoptosis in rats with Medtronic balloon-induced injury

作　　者：高虹 董少红 曾春苗 罗特丹 刘华东 

机构地区：[1]暨南大学第二临床医学院深圳市人民医院心内科,广东省深圳市518020

出　　处：《中国组织工程研究与临床康复》2010年第17期3148-3152,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:球囊扩张及支架置入后再狭窄、管腔丢失等问题制约了支架置入治疗的进一步发展,血管内膜增殖和凋亡在再狭窄中的作用及干预方法尚在积极探索中。目的:采用Medtronic球囊建立大鼠颈动脉球囊损伤模型,观察瑞舒伐他汀对球囊损伤大鼠颈动脉平滑肌细胞增殖及凋亡的影响。方法:将雄性SD大鼠随机数字表法分为球囊损伤组和治疗组。均采用Medtronic球囊建立大鼠颈动脉球囊损伤模型,并取球囊损伤组大鼠的右侧颈总动脉(未行球囊损伤)作为正常对照。治疗组于球囊损伤前3d始连续给予瑞舒伐他汀5mg/(kg·d)灌胃,球囊损伤组给予9g/L氯化钠溶液灌胃。术后7,14d取颈总动脉进行苏木精-伊红染色,免疫组织化学检测SMα-actin、增殖细胞核抗原,采用TUNEL法检测平滑肌细胞凋亡情况。结果与结论:与球囊损伤组比较,治疗组造模后14d,损伤血管新生内膜面积、新生内膜/中膜面积比值明显减少(P<0.05),管腔面积增加26%;增殖细胞核抗原阳性细胞率降低,凋亡阳性细胞率增高(P<0.05)。提示支架置入球囊损伤前给予瑞舒伐他汀可抑制大鼠颈动脉球囊损伤后新生内膜增生及新生内膜细胞的增殖,促进平滑肌细胞的凋亡,从而减少动脉新生内膜形成,抑制再狭窄。BACKGROUND：Restenosis and lumina loss limit further application of balloon extension and stent implantation.Effect of tunica intima proliferation and apoptosis in restenosis and the intervention method are exploring.OBJECTIVE：To investigate the influence of Rosuvastatin on the vascular smooth muscle cells proliferation and apoptosis in rats with carotid artery injury established by Medtronic balloon.METHODS：The male SD rats were randomly and equally divided into injury group and treatment group.Each rat was subjected to balloon injury on the left common carotid artery,and control artery without balloon injury on the right artery served as control group.Treatment group rats were given Rosuvastatin （dissolved in Nacl） 5 mg/kg per day 3 days before injury,while the injury group rats were given 9 g/L NaCl.At 7 and 14 days after injury,the common carotid arteries were harvested for HE staining.SM α-actin and proliferating cell nuclear antigen were detected by immunohistochemistry.In addition,smooth muscle cells apoptosis was detected by TUNEL.RESULTS AND CONCLUSION：The neointimal area and the area ratio of neointimal/media were decreased in treatment group significantly at 14 days compared with injury group （P 0.05）,and neointimal area increased by 26%;positive cell rate of proliferating cell nuclear antigen was decreased,but apoptosis cells were increased compared with the injury group （P 0.05）.Results showed that Rosuvastatin prior to balloon injury inhibited neointimal proliferation and neointimal cell proliferation following balloon injury,promoted smooth muscle cells apoptosis,ultimately reducing neointimal formation and inhibiting restenosis.

关 键 词：瑞舒伐他汀 颈动脉狭窄 疾病模型 动物 球囊 血管损伤 支架置入 

分 类 号：R318[医药卫生—生物医学工程]