甘草多糖对H22荷瘤小鼠的免疫调节作用  

Immunoregulatory function of Radix Glycyrrhizae polysaccharide in tumor-bearing mice

作　　者：李晓冰[1,2] 何小鹃[2] 刘彪[3] 徐荔[4] 鞠大宏[5] 姜淼[2] 吕爱平[2] 

机构地区：[1]上海中医药大学,上海201203 [2]中国中医科学院中医临床基础医学研究所,北京100700 [3]湖北大学生命科学学院,湖北武汉430062 [4]北京化工大学生命科学与技术学院,北京100029 [5]中国中医科学院中医基础理论研究所,北京100700

出　　处：《中西医结合学报》2010年第4期363-367,共5页Journal of Chinese Integrative Medicine

基　　金："十一五"国家科技支撑计划资助项目(No.006BAI08B01-01);国家自然科学基金资助项目(No.30902003);中国中医科学院课题基金资助项目(No.Z02116)

摘　　要：目的:建立H22荷瘤小鼠模型,观察甘草多糖对模型小鼠脾脏调节性T细胞(regulatory T cell,Treg)及脾淋巴细胞转化率的影响,探讨甘草多糖的免疫调节机制。方法:50只Balb/c小鼠随机分为正常组、模型组、环磷酰胺组、甘草多糖组和甘草多糖加环磷酰胺组。除正常组外,其余各组建立H22荷瘤小鼠模型。造模第2天各组开始给药,正常组和模型组腹腔注射生理盐水,环磷酰胺组腹腔注射环磷酰胺1周,甘草多糖组颈后皮下注射甘草多糖,连续14 d。取脾制备脾单细胞悬液,用流式细胞仪检测脾调节性T细胞,四甲基偶氮唑盐比色法检测脾淋巴细胞转化率。结果:模型组小鼠Treg细胞比例明显高于正常组,甘草多糖组Treg细胞比例低于模型组(P<0.01),甘草多糖组脾淋巴细胞转化率明显高于环磷酰胺组。甘草多糖与环磷酰胺无交互作用。结论:甘草多糖可能通过降低荷瘤小鼠Treg细胞的比例及提高脾淋巴细胞转化率而发挥抗肿瘤免疫调节作用。Objective：To observe the effects of Radix Glycyrrhizae polysaccharide on regulatory T cells（Treg） in spleen and lymphocyte transformation ratio in tumor-bearing mice so as to explore the mechanisms of its immunoregulatory function. Methods：Fifty BALB/c mice were randomly divided into normal group,untreated group,cyclophosphamide group,Radix Glycyrrhizae polysaccharide group and Radix Glycyrrhizae polysaccharide plus cyclophosphamide group.Except normal group,mice were subcutaneously implanted H22 tumor cells in the right axillary region.After 24 h,mice in normal and untreated group were subcutaneously injected with physiological saline,while mice in the cyclophosphamide group were intraperitoneally injected with cyclophosphamide and mice in Radix Glycyrrhizae polysaccharide group were subcutaneously injected with polysaccharide.Fourteen days later,Treg cells of spleen were detected by flow cytometry and lymphocyte transformation ratio was detected by methyl thiazolyl tetrazolium method. Results：The proportion of Treg cells was significantly higher in the untreated group than in the normal group, and was lower in the Radix Glycyrrhizae polysaccharide group than in the untreated group（P0.01）. Lymphocyte transformation ratio in the Radix Glycyrrhizae polysaccharide group was higher than that in the cyclophosphamide group.There was no interaction between Radix Glycyrrhizae polysaccharide and cyclophosphamide. Conclusion：Radix Glycyrrhizae polysaccharide can regulate the cellular immunity disorders of tumor-bearing mice by decreasing proportion of Treg cells and increasing spleen lymphocyte transformation ratio.

关 键 词：甘草多糖 T细胞 调节性 肝肿瘤 实验性 小鼠 

分 类 号：R285.5[医药卫生—中药学]