白介素-11对小鼠异基因骨髓移植后aGVHD的预防作用  

Prevention of Acute Graft-versus-host Disease by Interleukin-11 in a Murine Allogeneic Bone Marrow Transplantation Model

作　　者：庄强[1] 江松福[1] 梁彬[1] 俞康[1] 陈必成 

机构地区：[1]温州医学院附属第一医院血液科,325003 [2]温州市外科学重点实验室,325003

出　　处：《医学研究杂志》2010年第4期38-40,127,共4页Journal of Medical Research

基　　金：浙江省自然科学基金(Y207422);浙江省医药卫生科学研究基金(Y200710439);温州市科技局科技合作项目(H20090010)

摘　　要：目的评估白介素-11(Interleukin-11,IL-11)对小鼠异基因骨髓移植(allo-BMT)后急性移植物抗宿主病(aGVHD)的预防作用。方法用主要组织相容性抗原(MHC)完全不合的纯种近交系SPF级小鼠,雄C57BL/6(H-2b)为供鼠,雌性BALB/C(H-2d)为受鼠,建立allo-BMT/aGVHD动物模型,随机分3组:模型对照组、IL-11组(剂量为500μg/(kg.d)、环孢菌素A(CsA)组(剂量为10mg/(kg.d),观察其对aGVHD的预防作用。结果 IL-11组、CsA组受鼠生存时间均长于模型对照组(P<0.01);IL-11组、CsA组受鼠aGVHD临床表现和病理改变均较模型对照组明显轻。IL-11组、CsA组之间平均生存时间比较无统计学差异(P=0.901)。结论 IL-11可有效预防小鼠allo-BMT后aGVHD,减轻其症状和病理损害程度,显著延长平均生存时间。ObjectiveTo evaluate the prophylactic effect of interleukin-11（IL-11） on acute graft-versus-host disease （aGVHD） in a murine allogeneic bone marrow transplantation （allo-BMT） model. MethodsAcute GVHD was induced in male BALB/C（H-2d） mice by total body irradiation （TBI） followed by female allogeneic C57BL/6（H-2b）bone marrow and spleen cells transplantation. The experiment groups were designed as follows： control group, IL-11 （ 500μg/（kg·d） group, cyclosporine A （[CsA, 10mg/（kg·d）] group. The occurrence of aGVHD was assessed by signs of weight loss, diarrhea, ruffled fur, hunched posture and histologic changes of skin, liver and small intestines. ResultsThe survival time of IL-11 group and CsA group were prolonged as compared with that of the control group （P〈0.01）. aGVHD signs and histologic changes of IL-11 group and CsA group were milder than those of the control group. ConclusionIL-11 can be effective in prevention for aGVHD after allo-BMT in a murine model.It can decrease the aGVHD signs and histologic changes,and markly prolong mean survival time.

关 键 词：白介素-11 移植物抗宿主病 急性 异基因骨髓移植 小鼠 

分 类 号：R457.7[医药卫生—治疗学]