左归丸对免疫性卵巢早衰小鼠的治疗作用  

Treatment Effect of Zuogui Pill on Immune Premature Ovarian Failure Rats

作　　者：李红梅 钟志勇[2] 苗竹林 韦相才 范强 

机构地区：[1]广东省计划生育研究所,广州510600 [2]广东省医学实验动物中心,广州528248

出　　处：《医学研究杂志》2010年第4期43-46,共4页Journal of Medical Research

基　　金：广东省自然科学基金资助项目(07001360);广东省人口与计划生育资助项目(2008005)

摘　　要：目的通过对BALB/c小鼠多点注射透明带多肽,造成免疫性卵巢早衰模型,研究左归丸对免疫性卵巢早衰模型的治疗作用。方法 (1)建立小鼠免疫性卵巢早衰模型:以小鼠透明带3所合成透明带多肽为免疫原,以其免疫SPF级BALB/C雌性小鼠。以出现动情周期紊乱、卵巢细胞受损,表示造模成功。(2)造模成功后,将BALB/c小鼠随机分为模型组、左归丸高、中、低剂量组,泼尼松组、己烯雌酚组和对照组,共灌胃给药4周。结果模型组小鼠在第3～8周的体重与对照组比较,具有显著性差异(P<0.05)。模型组仅有16.7%(2/12)的动物维持正常的性周期,而空白对照组有91.7%(11/12)的动物维持正常的性周期。模型组小鼠卵巢中颗粒细胞、卵母细胞的凋亡率与对照组比较,具有显著性差异(P<0.05)。结论左归丸可能具有改善免疫性卵巢早衰小鼠症状的作用。ObjectiveTo explore the treatment effect of Zuogui pill （ZGP） on immune premature ovarian failure rats.MethodsFemale mice model of POF was established by multiple sites subcutaneous injection of zona pellucida 3,and treated with different dosage ZGP （low, middle and high）,with prednisone and diaethylstilbestrol as positive control. We detecteed apoptosis rate of granulosa and oocyte of murine ovaries by tunel method and observd the change of sexual cycle and weight. ResultsCompared with the control group,the weight of model group rat in the third to eighth weeks was significant difference （P〈0.05）. Only 16.7%（2/12）matained the sexual cycle in the model mice, 91.7%（11/12）in control group, 16.7% in low dosage ZGP, 25% in middle dosage ZGP and 50% in high dosage ZGP.Granulosa cell and Oocyte apoptosis rate in the model mice was higher.The differences were significant as compared with the low, middle and high dosage ZGP and control group（P〈0.05）. ConclusionZuogui pill may have some prevention and treatment action on POF.

关 键 词：左归丸 卵巢早衰 性周期 细胞凋亡 

分 类 号：R965.1[医药卫生—药理学]