猪瘟疫苗免疫反应低下猪CD3^+CD4^+和CD4^+CD25^+淋巴细胞亚群分析  

Analysis of CD3^+CD4^+ and CD4^+CD25^+ Lymphocyte Subsets of Pigs with Dumbing Immune Response to Classical Swine Fever Vaccine

作　　者：王惟[1,2] 杨知[1,2] 付明山[1,2] 郑海英[3] 李芳萍[3] 涂长春[2] 

机构地区：[1]吉林大学畜牧兽医学院,长春130062 [2]军事医学科学院军事兽医研究所,长春130062 [3]通辽市畜牧兽医科研所,内蒙古通辽028042

出　　处：《中国生物制品学杂志》2010年第4期378-381,共4页Chinese Journal of Biologicals

基　　金：国家科技支撑计划(2006BAD06A18)

摘　　要：目的分析猪瘟疫苗免疫反应低下猪CD3+CD4+和CD4+CD25+淋巴细胞亚群的分布。方法将接种过猪瘟疫苗的种母猪经ELISA和血清中和试验检测筛选出猪瘟病毒(CSFV)抗体阴性猪。从中挑选出15头作为研究对象,以15头抗体阳性猪作为对照。采集猪的抗凝血,分离外周血淋巴细胞(PBL),流式细胞术检测30头猪PBL中CD3+CD4+和CD4+CD25+淋巴细胞亚群的分布。结果CSFV抗体阴性猪PBL中CD3+CD4+细胞的比例(24.09%±1.29%)明显低于CSFV抗体阳性猪(37.49%±1.60%);CSFV抗体阴性猪PBL中CD4+CD25+细胞的比例(2.34%±0.20%)明显高于CSFV抗体阳性猪(1.64%±0.13%)。结论PBL中CD3+CD4+淋巴细胞亚群的减少及CD4+CD25+淋巴细胞亚群的增加,可能是导致猪瘟疫苗免疫反应低下的重要原因。Objective To analyze the distribution of CD3＋CD4＋ and CD4＋CD25＋ lymphocyte subsets in the pigs with dumbing immune response to classical swine fever（CSF）vaccine. Methods Fifteen CSF virus（CSFV）antibody-negative pigs were screened by ELISA and serum neutralizing antibody test from the female breeding pigs immunized with CSF vaccine, using 15 antibody-positive pigs as control. The anticoagulant blood of pigs was collected for isolation of peripheral blood lymphocytes（PBLs）in which the distribution of CD3＋CD4＋ and CD4＋CD25＋ lymphocyte subsets was determined by flow cytometry. Results The percentage of CD3＋CD4＋ lymphocyte subsets was significantly lower in PBLs of CSFV antibody-negative pigs（24. 09% ± 1. 29%）than in CSFV antibody-positive ones（37. 49% ± 1. 60%）, while that of CD4＋CD25＋ lymphocyte subsets was significantly higher in the former（2. 34% ± 0. 20%） than in the latter （1. 64% ± 0. 13%）. Conclusion The decrease of CD3＋CD4＋ and increase of CD4＋CD25＋ lymphocyte subsets in PBLs might be the important causes of dumbing immune response to CSF vaccine.

关 键 词：猪瘟病毒 外周血淋巴细胞 CD3+CD4+亚群 CD4+CD25+亚群 

分 类 号：R392-33[医药卫生—免疫学] S852.651[医药卫生—基础医学]