沙立度胺对大鼠子宫内膜异位组织中VEGF及TNF-α表达的影响  

Effect of thalidomide on the expression of VEGF and TNF-α in ectopic endometrial tissue in rats

作　　者：马芳[1] 黄学惠[1] 董文漪[1] 吴立涛[1] 

机构地区：[1]昆明医学院第一附属医院妇科,昆明650032

出　　处：《现代妇产科进展》2010年第3期194-197,共4页Progress in Obstetrics and Gynecology

基　　金：昆明医学院研究生创新基金资助课题(No:KM2008L45)

摘　　要：目的:探讨沙立度胺(thalidomide,THD)对大鼠子宫内膜异位组织中血管内皮生长因子(vascular endothelial growth factor,VEGF)和肿瘤坏死因子-α(tumornecrosis factor-alpha,TNF-α)表达的影响。方法:自体移植子宫内膜组织至腹膜,用SD大鼠建立子宫内膜异位症动物模型,3周后随机将建模成功的24只大鼠分为4组,每组6只,其中3组分别腹腔注射沙立度胺5mg/kg、20mg/kg和40mg/kg,模型对照组腹腔注射生理盐水,每天1次,连用4周。治疗结束后处死大鼠,获取异位子宫内膜组织。用免疫组化(SP)法测定VEGF和TNF-α在异位内膜的表达。结果:不同剂量THD给药组VEGF和TNF-α的表达均低于对照组(P<0.05),其中以沙立度胺40mg/kg组最为明显。结论:THD可以抑制VEGF和TNF-α表达,从而发挥抗EMs血管生成作用,且其对VEGF和TNF-α表达的影响具有量效关系。Objective：To explore the effect of thalidomide on the expression of vascular endothelial growth factor（VEGF）and tumor necrosis factor-α（TNF-α） in ectopic endometrial tissue in female rats with endometriosis（EMs）.Methods：Endometriosis was surgically induced in SD rats by transplanting an autologous fragment of endometrial tissue onto the inner surface of the abdominal wall.After 3 weeks＇ observation,24 female rats that successfully established EMs model were divided randomly into four groups：three groups（n=6,respectively） were given thalidomide（5mg/kg,20mg/kg,40mg/kg once a day,respectively）,and the model control group（n=6） were given normal saline（2ml）,which was injected intraperitoneally.Four weeks after treatment,rats were killed and ectopic uterine tissues were obtained.VEGF and TNF-α was detected in ectopic endometrium by immunohistochemical SP technique.Results：The expressions of VEGF and TNF-α were decreased in the endometriotic lesions in treatment group compared to the model group（P0.05）,with the most significant reduction in thalidomide 40mg/kg group.Conclusion：THD exists antiangiogenic effect in rat EMs by inhibit the expressions of VEGF and TNF-α in ectopic endometrium,and its inhibitory effect on the expressions of VEGF and TNF-α shows dose-effect relationship.

关 键 词：子宫内膜异位症 沙立度胺 血管内皮生长因子 肿瘤坏死因子-α 大鼠 Sprague-Dawley 疾病模型 动物 

分 类 号：R711.71[医药卫生—妇产科学]