金属锌离子对胆绿素的荧光增强作用  

Fluorescence Enhancement of Biliverdin by Zinc Ion

作　　者：郑文杰[1] 胡学旺[1] 冯德雄[1] 

机构地区：[1]暨南大学化学系

出　　处：《暨南大学学报（自然科学与医学版）》1998年第5期63-66,共4页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：广东省自然科学基金

摘　　要：研究了不同条件下Ｚｎ２＋对胆绿素（ｂｉｌｉｖｅｒｄｉｎ，ＢＶ）荧光性质的影响作用。发现在无水乙醇、甲醇、ＮＨ３－ＮＨ４Ｃｌ缓冲体系和ＮａＯＨ水溶液中，Ｚｎ２＋均对ＢＶ有显著的荧光增强作用。在ＮａＯＨ水溶液中，在表面活性剂（ｃｅｔｙｌｔｒｉｍｅｔｈｙｌａｍｍｏｎｉｕｍｂｒｏｍｉｄｅ，ＣＴＡＢ）存在下，Ｚｎ２＋与ＢＶ的摩尔比率为２，荧光增强作用达到最大值。借助这一荧光增强技术，可以在室温下使胆绿素水溶液产生特征强荧光（λｅｘ＝４６５ｎｍ，λｅｍ＝５２５ｎｍ）。The use of metal ions for the fluorescence enhancement of biliverdin in sever- al media was investigated. It was found that adding zinc salt increased fluorescent in- tensity of biliverdin remarkablely in methanol, ethanol, NH3 - NH4Cl buffer and aqueous alkaline solutions etc. The fluorescence enhancement reached its maximum when mole ratio of zinc ion to biliverdin was 2 in the presence of surfactant cetyltrimethyl ammonium bromide (CTAB) in aqueous alkaline solution . By means of the fluorescent enhancement, the specific strong fluorescence of biliverdin(λex= 465nm, λem= 525nm ) was obtained in aqueous solution at room temperature.

关 键 词：胆绿素 荧光增强作用 锌离子 胆汁色素 

分 类 号：Q486[生物学—生理学] Q593.9