老年性记忆减退大鼠内侧隔核-斜角带NGFR和NOS神经元各亚群的改变  

The Changes of Subpopulation of NOS, NGFR Neuron in Septum Medialis-Diagonal Band Complex of the Senile Memory Deficiency Rat

作　　者：王怀星[1] 姚志彬[1] 顾耀铭[1] 陈以慈[1] 

机构地区：[1]中山医科大学解剖教研室

出　　处：《暨南大学学报（自然科学与医学版）》1998年第6期104-104,共1页Journal of Jinan University(Natural Science & Medicine Edition)

摘　　要：本研究采用健康雄性ＳＤ大鼠，青年组（３月龄）２０只，老年组（２６月龄）４０只，通过Ｍｏｒｉｓ水迷宫行为学测试，将老年大鼠分为老年记忆减退组（简称老年减退组）和老年记忆正常组（简称老年正常组）。用免疫细胞化学、组织化学双标技术定量分析内侧隔核－斜角带（ｓｅｐｔｕｍｍｅｄｉａｌｉｓ－ｄｉａｇｏｎａｌｂａｎｄｏｆＢｒｏｃａ，ＳＭ－ＤＢ）中神经生长因子受体（ｎｅｒｖｅｇｒｏｗｔｈｆａｃｔｏｒｒｅｃｅｐｔｏｒ，ＮＧＦＲ）、一氧化氮合酶（ｎｉｔｒｉｃｏｘｉｄｅｓｙｎｔｈａｓｅ，ＮＯＳ）阳性神经元各亚群。老年减退组大鼠和老年正常组大鼠相比较，ＳＭ、斜角带垂直支（ｖｅｒｔｉｃａｌＤＢ，ｖＤＢ）、斜角带水平支（ｈｏｒｉｚｏｎａｌＤＢ，ｈＤＢ）中ＮＧＦＲ－ＮＯＳ双标神经元数量明显下降（Ｐ＜００１），其下降幅度为２８８４％，２３３３％和２０３５％；ＮＧＦＲ单标神经元在ＳＭ、ｖＤＢ和ｈＤＢ中也呈明显下降，其下降值为４０４３％，４３２４％，３３０６％；ＮＯＳ单标神经元的数量较老年正常组有显著下降（Ｐ＜００５），下降幅度分别为１７２４％，２０００％和１８６０％。相关分析表明，受试大鼠逃避潜伏期与双标神经元、ＮＧＦ?male healthy SD rats, 20 of young (3 months) and 40 of old (26 months ), were examined with Morris water maze. The old were divided into senile memory deficiency rats (SMD) and senile normal rats (SN).The subpopulations of NOS and NGFR neuron of SM-DB complex were analysis qualitively and quantitatively. There were 3 kinds of neuron in NGFR-NOS double labeled preparation. The number of NGFR-NOS double labeled, NGFR single labeled and NOS single labeled neuron is SM-DB complex of SMD had a significant decrease than that of SN. That of SN and the young no significant difference. The reduction of NGFR single labeled in SM-DB of SMD is most obvious in three kinds of neurons. The decrease range of the NOS single labeled of SMD was smaller than that of others. There were significant correlations between the mean escape latency of the examined rats and the number of double labeled, the NGFR single labeled, and NOS single labeled neuron. Although the double labeled, the NGFR single labeled and NOS single labeled.

关 键 词：记忆减退 内侧隔核 斜角带 神经元 NGFR NOS 

分 类 号：R338.64[医药卫生—人体生理学]