不同浓度胰岛素对Ishikawa细胞IGFBP-1 mRNA表达的影响  

The Influence of Insulin on Expression of IGFBP-1 mRNA in Ishikawa Cells

作　　者：韩艳[1] 黄筱金[1] 肖帆[2] 彭清妹[2] 

机构地区：[1]南昌大学第一附属医院辅助生殖科,南昌330006 [2]井冈山大学医学院妇产科,江西吉安343000

出　　处：《江西医学院学报》2009年第12期42-45,共4页Acta Academiae Medicinae Jiangxi

摘　　要：目的体外研究胰岛素对子宫内膜上皮细胞胰岛素样生长因子结合蛋白-1(IGFBP-1)mRNA表达的影响。方法Ishikawa细胞培养液中分别添加雌二醇(E2)200pg/mL以模拟增殖晚期正常子宫内膜上皮细胞(A组),E2200pg/mL和孕酮(P)20ng/mL模拟分泌中期(B组);再将A、B组分别予0、10、30、100mU/L的胰岛素水平供培养。采用RT-PCR法检测Ishikawa细胞IGFBP-1 mRNA的表达情况。结果①A、B组IGFBP-1 mRNA表达均为阳性,B组表达高于A组(P<0.0001)。②A、B组不同浓度下,IGFBP-1 mRNA的表达有统计学意义(P<0.0001),且随胰岛素浓度的升高,IGFBP-1 mRNA表达量下降。③A、B组间30mU/L与100mU/L胰岛素浓度的IGFBP-1 mRNA表达无统计学意义(P>0.05)。结论①Ishikawa细胞分泌中期分泌IGFBP-1较增殖晚期增加,孕激素可上调IGFBP-1的表达。②Ishikawa细胞IGFBP-1 mRNA的表达不依赖于胰岛素的作用,但胰岛素可抑制IGFBP-1 mRNA,具有浓度依赖性。③高浓度胰岛素可能可以拮抗孕激素对IGFBP-1 mRNA表达的促进作用。Objective To study the influence of insulin on expression of IGFBP-1 in Ishikawa cell.Method 200 pg/L ESTRADIOL （E2） was added in Ishikawa cell medium to simulate the late endometrial proliferation （group A）,and 200 pg/L estradiol （E2） and 20 ng/L progesterone （P） was added to simulate the endometrial mid-secretion （group B）.Simultaneously,group A and B were respectively treated with 0 mU/L,10 mU/L,30 mU/L,100 mU/L insulin,RT-PCR was used to detect the IGFBP-1 mRNA expression after 24 h of culture. Results ①The IGFBP-1 mRNA expression were positive in both group A and B,with group B expressed more than group A （P0.0001）.②As insulin concentration ascended,IGFBP-1 mRNA expression decreased significantly in both group A and B（P0.001）.③There was no significant difference in IGFBP-1 mRNA expression in both group A and group B when treated with 30 mU/L and 100 mU/L insulin （P0.05）.Conclusion ①Progesterone can increase the expression of IGFBP-1 mRNA in Ishikawa cells and the expression of IGFBP-1 mRNA is more highly in the endometrial mid-secretion than in the late endometrial proliferation.②IGFBP-1 mRNA expression is not dependent on insulin,but insulin can suppress IGFBP-1 mRNA expression in a dose-dependent manner.③High concentration of insulin may be able to inhibit the of IGFBP-1 mRNA expression stimulated by progesterone.

关 键 词：多囊卵巢综合征 ISHIKAWA细胞 胰岛素样生长因子结合蛋白-1 雌二醇 孕酮 

分 类 号：R711.75[医药卫生—妇产科学]