实验犬环杓后肌功能重建的组织化学研究及图像分析  

HISTOCHEMISTRY STUDY AND IMAGE ANALYSIS ON RECOVERY OF POSTERIOR CRICOARYTENOID MUSCLE'S FUNCTION IN THE EXPERIMENTAL DOG

作　　者：黄秀琴[1] 蒋淑萱[1] 吴平[1] 张德芳[1] 王正强[1] 梁伟平 陈贤明[1] 马丽梅[1] 李坪[1] 

机构地区：[1]昆明医学院组织学胚胎学教研室,**寄生虫学教研室,昆明650031*昆明医学院附属二院耳鼻咽喉科

出　　处：《解剖学报》1998年第4期430-434,I014,I015,共7页Acta Anatomica Sinica

基　　金：云南省自然科学基金

摘　　要：为了确定并比较实验犬两种不同术式：Ⅰ．颈袢胸骨舌骨肌支肌蒂移植术；Ⅱ．副神经胸锁乳突肌支肌蒂移植术，治疗声带麻痹的效果。建立单侧联合性声带麻痹动物模型，分别用Ⅰ、Ⅱ两种术式移植神经肌蒂于失神经的环杓后肌（ＰＣＡＭ）。术后４个月取各组ＰＣＡＭ做超微结构检查，并做光镜组织化学：乙酰胆碱酯酶（ＡＣｈＥ）、琥珀酸脱氢酶（ＳＤＨ）、三磷酸腺苷酶（ＡＴＰａｓｅ）、糖原（ＰＡＳ）反应。用图像分析定量并测示肌纤维直径。结果发现两种术式都能使ＰＣＡＭ恢复功能，第Ⅱ种术式组织化学结果优于第Ⅰ种术式。Comparing curative effects of two surgical procedures of transplanting neuromuscular pedicles (NMP) into posterior cricoarytenoid muscles (PCAM) in the experimental dog. An animal model of unilateral combined vocal cord paralysis (VCP) was established, the ansa cervicalis sternastoideus and the accessory nerve sternocleidonastoideus were separately transplanted into uninnervated PCAM. Fourth month after operation, PCAM form all the groups were detected with AChE、SDH、ATP ase and PAS histochemistry methods and the results were quantitated by image analysis. The fiber diameter was measured and ultramicroscopic structure was observed. Result: Above two surgical procedures of NMP transplantation are effective and available on curing VCP. Both can restore the function of PCAM, and the second procedure is better than the first.

关 键 词：声带麻痹 环杓后肌 神经肌蒂 移植术 

分 类 号：R767.64[医药卫生—耳鼻咽喉科]