准弹性光散射测量机理及其应用  

Principle and Application of Quasi-elastic Light Scattering(QELS) Technique

作　　者：沈子威 齐娟 宋传琳[2] 黄美珍 

机构地区：[1]生物科学与技术系 [2]水利水电工程系 [3]精密仪器系

出　　处：《清华大学学报（自然科学版）》1990年第3期93-100,共8页Journal of Tsinghua University(Science and Technology)

摘　　要：准弹性光散射技术是生物学中一种新颖的技术，具有测量速度快，精度高等 优点。准弹性光散射测量原理是基于经典的多普勒效应，利用 Ｒａｙｌｅｉｇｈ－Ｇａｎｓ－Ｄｅｂｙｅ （ＲＧＤ）近似方法推导出微粒运动速度和频率变化之间关系。对生物微粒（精子与四膜 虫）进行测量表明 ＲＧＤ近似方法完全适用。The basic principles of light scattering which apply to the QELS technique and particle velocimetry are introduced in this paper. This is followed by a rigorous treatment of light scattering from extended particles such as biological cells.A computationally convenient form of the RGD approximation is introduced.The RGD approximation is used to treat structure and dynamic effects commonly encountered in light scattering from cells.The RGD theory is a satisfactory method for the analysis of QELS data. The motion of spermatozoa and pyriformis are measured by the QELS technique

关 键 词：生物微粒 光散射 测量 准弹性 

分 类 号：Q-331[生物学]