黄芪多糖对成骨细胞增殖分化和结构的影响  

Effect of Astragalus polysaccharide on the proliferation differentiation and structure of osteoblasts

作　　者：刘豫蓉[1] 张朝良[1] 孔祥丽[1] 陈思秀[1] 李小玉[1] 

机构地区：[1]口腔疾病研究国家重点实验室,四川大学,四川成都610041

出　　处：《国际口腔医学杂志》2010年第2期133-137,共5页International Journal of Stomatology

基　　金：四川省科技支撑计划基金资助项目(2009FZ0062)

摘　　要：目的探讨黄芪多糖对新生大鼠颅骨成骨细胞增殖和分化以及结构的影响。方法体外培养新生大鼠颅骨成骨细胞,检测其在质量浓度分别为0.08、0.1、0.2、0.4g·L-1的黄芪多糖培养基中的增殖和分化与结构。结果当黄芪多糖的质量浓度为0.1g·L-1时,甲噻唑四唑氮法所测其光密度值和碱性磷酸酶活性高于对照组,成骨细胞能保持良好的表面结构和超微结构。结论适宜质量浓度的黄芪多糖水溶液在短期内对体外成骨细胞的增殖与分化具有一定的促进作用。Objective To investigate the effect of Astragalus polysaccharide（APS） on the proliferation differentiation and structure of neonate rat calvarium osteoblasts in vitro.Methods Osteoblasts were cultured in vitro with APS of 0.08,0.1,0.2,0.4 g·L-1.Methyl thiazolyl tetrazolium,capacity of alkaline phosphatase and cell structure were detected to determine osteoblasts proliferation and differentiation.Results APS concentration of 0.1 g·L-1 can improve the osteoblasts proliferation rate and alkaline phosphatase activity and have no negative influence on the structure of cells.Conclusion APS with proper mass concentration in culture may promote osteoblasts proliferation and differentiation.

关 键 词：黄芪多糖 成骨细胞 增殖 分化 结构 

分 类 号：R285[医药卫生—中药学]