抗氟甲砜霉素多克隆抗体的制备与鉴定  

Preparation and identification of polyclonal antibody against florfenicol

作　　者：褚文雷[1] 崔杰[2] 马莺[2] 李滨胜[3] 

机构地区：[1]哈尔滨工业大学生命科学与工程系,哈尔滨150001 [2]哈尔滨工业大学食品科学与工程学院,哈尔滨150090 [3]黑龙江省森林植物园引种驯化研究中心,哈尔滨150040

出　　处：《黑龙江大学自然科学学报》2010年第2期242-245,259,共5页Journal of Natural Science of Heilongjiang University

基　　金：哈尔滨市科技攻关资助项目(2007AA6CN099)

摘　　要：为建立氟甲砜霉素的免疫分析方法,采用混合酸酐法合成氟甲砜霉素人工抗原(FF-HS-BSA)和包被抗原(FF-HS-OVA),并利用紫外吸收光谱及红外光谱扫描方法检测到人工合成抗原偶联成功。利用合成的人工抗原免疫5只6～8周龄BALB/c小鼠,免疫8次后得到氟甲砜霉素抗血清。利用间接非竞争酶联免疫吸附测定法检测抗体效价为1∶6400,可用于进一步实验研究。In order to establish the immunoassay method of florfenicol （FF）, FF was coupled with carrier protein Bovine Serum Albumin（BSA） and Ovalbumin（OVA） to prepare complete antigen FF-HS-BSA and FF-HS-OVA by mixed anhydride （MA） reaction. UV and IR methods were used to identify synthetic antigen which results showed that the reaction was successed. Five BALB/C mice, 6 -8 weaks old,were immunized with FF-HS-BSA, and the polyclonal antibody（pAb） after 8 times immunized was got. The titer of pAb was detected by indirect ELISA, the titer of FF antiserum was 1 ： 6 400,it could be used in the next study.

关 键 词：氟甲砜霉素 人工抗原 多克隆抗体 ELISA 

分 类 号：S852.43[农业科学—基础兽医学]