HPLC-ELSD测定三黄合剂中黄芪甲苷的含量  

Determination of Astragaloside IV in Sanhuang Mixture by HPLC-ELSD

作　　者：刘庆丰[1] 李中东[1] 刘珏[1] 施孝金[1] 钟明康[1] 

机构地区：[1]复旦大学附属华山医院临床药学室,上海200040

出　　处：《中国现代应用药学》2010年第4期355-358,共4页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立三黄合剂中黄芪甲苷含量测定的HPLC方法并制定该制剂的质量控制指标。方法采用DiamonsilC18柱，流动相为乙腈-水（35：65），流速：1．0mL·min-1；漂移管温度为95℃，氮气流速为3．0L·min^-1。结果进样量在0．25～5．00μg内，黄芪甲苷浓度的对数值IgC与峰面积积分的对数值IgA之间呈良好的线性关系，r=0．9993。结论本法操作简便，准确，可用于三黄合剂的质量控制。OBJECTIVE To establish a method using HPLC coupled with ELSD for determination of astragaloside IV in Sanhuang mixture and formulate a standard for qulity control. METHODS A Diamonsil C18 column（200 mm×4.6 mm, 5μm） was used, acetonitrile-water （35：65） was used as mobile phase and the flow rate was 1.0 mL·min-l. The temperature of drift tube for ELSD was 95℃ and the flow rate of nitrogen was 3.0 L.min-1. RESULTS The linear range for astragaloside IV was 0.25-5.00 μg （r=0.999 3）, the RSDs of intra- and inter-day were less than 2.0%. CONCLUSION The method is simple, accurate and suitable for quality control of Sanhuang mixture.

关 键 词：三黄合剂 黄芪甲苷 高效液相色谱-蒸发光散射检测器 含量测定 

分 类 号：R917.101[医药卫生—药物分析学]