酚红分光光度法测定替米沙坦的含量  

Spectrophotometric Determination of Telmisartan with the Reagent Phenol Red

作　　者：谭蓉[1] 秦宗会[2] 马新会[2] 谭世龙[2] 刘於生[2] 罗华生[2] 

机构地区：[1]太极集团涪陵制药厂有限公司,涪陵408000 [2]长江师范学院化学及环境科学系,涪陵408100

出　　处：《理化检验（化学分册）》2010年第4期390-392,395,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：重庆市教委科研(KJ081301);重庆市涪陵区科委科技计划(2008-43);重庆市高等学校优秀中青年骨干教师资助计划[渝教人(2005)2号];长江师范学院科研(07Jkg50)资助

摘　　要：在pH为7.9的B-R缓冲体系中,酚红与替米沙坦作用,形成离子缔合物,溶液颜色发生改变,其最大显色和褪色波长分别为558,426 nm,替米沙坦的浓度与溶液的增色或褪色呈线性关系,建立了分光光度法测定替米沙坦的含量。在最大显色和褪色波长处,替米沙坦的浓度分别在6.50×10^(-7)～1.35×10^(-5)mol·L^(-1)和7.50×10^(-7)～1.00×10^(-5)mol·L^(-1)范围内遵守比耳定律,摩尔吸光率分别为6.59×10~4,2.12×10~4L·mol^(-1)·cm^(-1),检出限(3δ)分别为1.72×10^(-7),8.53×10^(-7)mol·L^(-1)。用于血样、尿样和药品中替米沙坦的测定,相对标准偏差(n=11)在0.72%～2.56%之间。In the B-R buffer solution of pH 7.9,an ion-association complex was formed by the reaction of telmisartan with phenol red,having its positive maximum absorption wavelength at 558 nm and the negative maximum absorption wavelength at 426 nm.Ranges of linearity and values of apparent molar absorptivity were found in the positive absorption region and in the negative absorption region were 6.50×10^-7 -1.35×10^-5 mol·L^-1,7.50×10^-7 -1.00×10^-5mol·L^-1,and 6.59×10^4L·mol^-1·cm^-1,2.12×10^4L·mol^-1·cm^-1 respectively.The detection limits（3δ） of the method found were 1.72×10^-7 mol·L^-1 and 8.53×10^-7 mol·L^-1 respectively.The proposed method was applied to the determination of telmisartan in the samples of medicine, human serum and human urine,giving values of RSD（n=11） in the range of 0.72%-2.56%.

关 键 词：酚红 替米沙坦 分光光度法 

分 类 号：O657.32[理学—分析化学]