酶催化光度法测定异丙肾上腺素  

Enzymatic Catalytic Photometric Determination of Isoprenaline

作　　者：陈亚红[1] 田丰收 庞纪磊[1] 

机构地区：[1]周口师范学院化学系,周口466000 [2]河南百年康鑫药业有限公司,郸城477150

出　　处：《理化检验（化学分册）》2010年第4期422-424,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：河南省高校科技创新人才支持计划资助项目(No.2009HASTIT034);河南省教育厅自然科学基金资助项目(No.2008A150029)

摘　　要：试验表明:在pH 9.5氨水-氯化铵缓冲介质中,牛血红蛋白对过氧化氢与酸性络蓝K的氧化还原反应的催化作用因异丙肾上腺素的存在而被抑制,而且其抑制率与异丙肾上腺素的浓度在8.1×10^(-8)～1.2×10^(-5)mol·L^(-1)范围内呈线性关系,其检出限(3S/N)为2.2×10^(-9)mol·L^(-1)。据此,提出了一种简单而灵敏的测定异丙肾上腺素的酶催化光度法。此方法已用于盐酸异丙肾上腺素注射液中异丙肾上腺素含量的测定,并以此样品为基体用标准加入法作回收试验,测得回收率在92.8%～105.4%之间,测定值的相对标准偏差(n=5)在2.6%～5.8%之间。It was found that in an ammoniacal buffer medium of pH 9.5,the catalytic effect of bovine hemoglobin on the redox reaction between hydrogen peroxide and acid chrome blue K was inhibited by the presence of isoprenaline,and that linear relationship was kept between values of rate of inhibition and concentration of isoprenaline in the range of 8.1×10^-8-1.2×10^-5 mol·L^-1 with its detection limit（3S/N） of 2.2×10^-9 mol·L^-1.On these basis,a sensitive and simple photometric method for determination of isoprenaline was proposed. The proposed method was used for the determination of isoprenaline in its injections and recovery of the method was tested by standard addition method using these samples as matrixes.Values of recovery found were ranged from 92.6%to 105.4%with values of RSD＇s（n=5） in the range of 2.6%-5.8%.

关 键 词：酶催化光度法 异丙肾上腺素 牛血红蛋白 

分 类 号：O657.32[理学—分析化学]