药效差示血清色谱法研究红花的药效物质基础  

Study on Effective Substance Basis of Carthamus Tinctorrius L by Differential Efficacy of Serum Chromatography

作　　者：乔逸[1] 杨志福[1] 田云[1] 杨静[1] 贾艳艳[1] 文爱东[1] 

机构地区：[1]第四军医大学西京医院药剂科,西安710033

出　　处：《中国药师》2010年第5期624-626,共3页China Pharmacist

基　　金：国家自然科学基金资助项目(编号:30672662)

摘　　要：目的:应用药效差示血清色谱法探索红花的药效物质基础。方法:采用HPLC指纹图谱分析方法,比较红花提取物及红花阴性提取物最大药效时刻、最小药效时刻的含药血清色谱图,探索红花在正常大鼠体内的药效物质基础。结果:红花提取物中出现了一个与药效强弱有关的物质峰,红花阴性提取物中既无药效也无此物质峰。结论:HSYA是红花的药效物质基础;药效差示血清色谱法是一种研究中药药效物质基础的新方法。Objective ： To investigate the effective substance of Carthamus tinctorrius L by differential efficacy of serum chromatography. Method： HPLC fingerprint analytical method was used to investigate the effective substance of Carthamus tinctorrius L in rats, comparing serum containing drug chromatogram of maximum efficacy time with minimum efficacy time between safflower extract and safflower negative extract. Result： A peak has been discovered in safflower extract which was changed with efficacy, safflower negative extract had no efficacy nor the peak of HSYA. Conclusion ： HSYA is the most effective substance of Carthamus tinctorrius L. Differential efficacy of serum chromatography is a new method of studying effective substance of Chinese herbal formula.

关 键 词：红花 药效差示血清色谱 指纹图谱 

分 类 号：R285.6[医药卫生—中药学]