基于均匀设计的祛湿化瘀方防治脂肪肝不同作用环节的主效应中药剖析  

Research of effects of main herbs in Qushi Huayu Decoction on adiposis hepatica based on uniform design

作　　者：陈少东[1,2] 冯琴[1] 彭景华[1] 许丽莉[1] 胡义扬[1] 

机构地区：[1]上海中医药大学附属曙光医院上海中医药大学肝病研究所,肝肾疾病病证教育部重点研究室,上海市高校中医内科学E-研究院,上海201203 [2]厦门大学医学院,厦门361005

出　　处：《中华中医药杂志》2010年第5期677-680,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.30672635);上海市优秀学科带头人计划项目(No.06XD14018);上海市重点学科建设项目(No.Y0302);上海高校创新团队建设项目(第1期)~~

摘　　要：目的:以对实验性脂肪肝有显著防治作用的祛湿化瘀方为切入点,探索中药复方多途径、多环节药理作用的相应物质基础的研究方法。方法:采用CCl4复合高脂低蛋白饮食诱导的大鼠脂肪肝模型,运用"均匀设计法",根据复方中的5味中药(茵陈、栀子、虎杖、田基黄、姜黄),选用U11(1110)表进行组方设计所得10种组合进行干预,分别以肝甘油三酯(triglyceride,TG)、血清丙氨酸氨基转移酶(alanine aminotransferase,ALT)、丙二醛(malonyl-dialdehyde,MDA)为不同病理环节的考察指标,根据回归方程获得针对上述不同指标所提示的最佳效应中药或某种配伍组合,并根据其提示的效应中药或组合,重新分组实验进行验证。结果回归方程显示,降低肝脏TG的最佳效应组合是茵陈0.07g、田基黄0.05g、栀子0.17g、姜黄0.03g,降低ALT的最佳效应组合是茵陈0.33g、虎杖0.25g、田基黄0.25g、姜黄0.17g。而针对MDA未获得回归方程。验证实验证实了上述"筛选"结果的可靠性。结论:①均匀设计是可靠的剖析某一病理环节主效应中药或配伍组合的分析方法;②在整体实验水平,针对某一指标环节的最佳效应中药仍以中药配伍组合方式出现,也说明了中药配伍复方针对复杂病理变化的科学性。Objective：To investigate the analysis method of basic material for multi-channels and multi-links pharmacologic action of complex prescription on basis of study effect of Qushi Huayu（QSHY） Decoction on adiposis hepatica.Methods：The adiposis hepatica model was induced by CCl4 with high fat and low protein diet.Apply the uniform design U11（1110） form for QSHY Decoction.Different decoctions groups were added with medicine respectively.The folliowing indexes were detedted：triglyceride（TG）,alanine aminotransferase（ALT） and malonyl dialdehyde（MDA）.Results：The content of TG decreased markedly in groups based on Artemisia capillaris Thunb 0.07g,Gardenia jasminoides Ellis 0.17g,hypericum japonicum Thunb.0.05g and curcuma 0.03g.While the activity of ALT decreased markedly in groups based on Artemisia capillaris Thunb 0.33g,Giant knotweed rhizome 0.25g,hypericum japonicum Thunb.0.25g and curcuma 0.17g.The QSHY Decoction group can decrease the content of MDA.Conclusion：①Uniform design was a reliable method for main effects Chinese medicine analysis of a pathological aspect or for compatibility analysis.②The results showed that the Chinese medicine produced the best effect as the compatible forms,it indicated the compatibility of Chinese medicine complex prescription was scientific.

关 键 词：祛湿化瘀方 脂肪肝 均匀设计 效应中药 

分 类 号：R285.5[医药卫生—中药学]