舒脉胶囊对血管紧张素Ⅱ诱导的血管平滑肌细胞增生及NO、SOD、MDA的影响  

Influence in Shumai Capsule on Ang Ⅱinduced vascular smooth muscle dell proliferation and level of NO,SOD,MDA

作　　者：董建勋[1] 余胜[2,3] 路广林[2] 郝钰[2] 张美吉[2] 朱朝军[1] 

机构地区：[1]首都医科大学附属北京中医医院,北京100010 [2]北京中医药大学,北京100029 [3]安徽医科大学第一附属医院,合肥230000

出　　处：《中华中医药杂志》2010年第5期752-754,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：北京市中医药科技发展基金资助项目(No.JJ2005-13)~~

摘　　要：目的:观察舒脉胶囊及其拆方药物血清对血管紧张素Ⅱ(AngⅡ)诱导的血管平滑肌细胞(VSMC)增生以及细胞培养上清液中一氧化氮(NO)、超氧化歧化酶(SOD)、丙二醛(MDA)的影响。方法:组织贴块法进行血管平滑肌细胞培养,应用血清药理学的方法,AngⅡ10-7mol/L作为刺激因子,将药物血清分为舒脉胶囊全方组、活血化瘀拆方组和补脾益肾拆方组,MTT比色法测OD值,生化试剂盒检测NO、SOD、MDA水平。结果:舒脉胶囊全方组和活血化瘀拆方组的OD值低于AngⅡ组和补脾益肾拆方组(P<0.01);AngⅡ组、补脾益肾拆方组NO和MDA量低于空白组、舒脉胶囊全方组和活血化瘀拆方组(P<0.01),而SOD水平高于上述3组(P<0.01)。结论:舒脉胶囊具有抑制AngⅡ诱导的血管平滑肌细胞增殖作用,并能促进NO和MDA的分泌,限制SOD的表达。而且舒脉胶囊全方和活血化瘀拆方作用更明显。Objective：To observe the impact of the medicated serum of Shumai Capsule and its decomposing on vascular smooth muscle cells（VSMC） proliferation induced by angiotensinⅡ（AngⅡ） and level of NO,SOD,MDA in cell culture supernatant.Methods：Tissue explant method was used for cultivating vascular smooth muscle cells,serum pharmacology was used,AngⅡ10-7mol/L as a stimulating factor,medicared serum divided into Shumai Capsule group,Huoxue Huayu decomposing group（group 1） and Bupi Yishen decomposing group（group 2）,MTT colorimetric was used to test OD values,biochemical detection kit was used to test NO,SOD,MDA levels.Results：The OD value of Shumai Capsule and group1 had significant difference from AngⅡ group and group2（P〈0.001）;NO level of AngⅡgroup and group2 was lower than that of the control group,Shumai Capsule group and group1（P〈0.001）;SOD level of AngⅡgroup,group2 was higher than that of the control group,Shumai Capsule group and group1（P〈0.001）;MDA level of AngⅡ group,group2 was lower than that of the control group,Shumai Capsule group and group1（P〈0.001） too.Conclusion：Shumai Capsule group and group 1 had the effect of inhibiting cell proliferation.by promoting the secretion of NO and MDA,limiting the expression of SOD.The inhibitory effect of Shumai Capsule and group2 were the best.

关 键 词：舒脉胶囊 血管紧张素Ⅱ 血管平滑肌细胞 一氧化氮 超氧化歧化酶 丙二醛 

分 类 号：R285.5[医药卫生—中药学]