温郁金醚提物中二萜类化合物C体外对人胃癌细胞的抑制作用  

Effect of diterpenoid C from Radix Curcumae on proliferation of human gastric cancer cells SGC-7901

作　　者：金海峰[1] 吕宾[1] 陈喆[1] 马忠俊[2] 

机构地区：[1]浙江中医药大学附属第一医院,杭州310006 [2]浙江大学药学院现代中药研究所,杭州310058

出　　处：《中华中医药杂志》2010年第5期783-786,共4页China Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:研究温郁金醚提物中二萜类化合物C体外对人胃癌细胞(SGC-7901)的抑制作用。方法:以含10%胎牛血清的RPMI1640为培养基,置37℃、体积分数为5%的CO2培养箱中培养SGC-7901细胞;将温郁金醚提物中二萜类化合物C溶于二甲基亚砜(Dimethyl sulfoxide,DMSO),配成10mg/mL母液;以顺铂(cis-platinum complexes,DDP)为阳性对照药物,采用噻唑蓝(MTT)比色法检测两种药物在10,30,40,70μg/mL浓度时对SGC-7901细胞增殖的影响;用倒置相差显微镜观察化合物C在40,55,70μg/mL浓度、24,48h对SGC-7901细胞的作用效果;用流式细胞仪检测化合物C在40,55,70μg/mL浓度对SGC-7901细胞周期分布的影响及其凋亡率。结果:MTT法显示化合物C对SGC-7901在较低浓度时的抑制率与阳性对照组相似,在40,70μg/mL浓度时的抑制率与对照组(DDP组)相比差异有统计学意义(P<0.05)。倒置相差显微镜下观察发现早期的凋亡细胞形态接近正常,核形态基本正常;晚期的凋亡细胞体积缩小或呈圆形,核固缩或碎裂,呈一定的时间和浓度依赖性。流式细胞术(flowcytometry,FCM)显示:温郁金二萜类化合物C能阻滞SGC-7901细胞于G0/G和S期,减少G2/M的比例。结论:温郁金醚提物中二萜类化合物C对胃癌SGC-7901细胞的增殖有显著的抑制作用,阻滞其细胞周期,抑制细胞增殖,是温郁金二萜类化合物C发挥抗肿瘤作用的机制之一。Objective：To study the biological effects of new monomer 3 from Radix Curcumae on proliferation of human gastric cancer cells SGC-7901.Methods：Uing fluorescence microscope to observe the impact on SGC-7901 cells with different concentrations of the new monomer 3 at different times,using MTT assay to observe the growth inhibition rate of SGC-7901 cells,using flow cytometry to observe the apoptosis rate and cell cycle distribution of SGC-7901 cell.Results：Pronounced apoptosis had been found by fluorescence microscopy,apoptosis had a certain amount of time-dependent and concentration-dependent;Early apoptotic cells near normal morphology,normal nuclear morphology;late apoptotic cells＇size shrink or round in shape,nuclear pyknosis or fragmentation.MTT method showed that the new monomer 3 had significantly inhibition on SGC-7901 cells,the half inhibition rate（IC50） was 30.14μg/mL.Flow cytometry（FCM） showed that：the new monomer 3 can increase the SGC-7901 cells in the S phase and G0/G1 and reduce it in G2/M.The change had time and concentration dependence;The apoptotic rates of dosing unit（24h,48h） compared with the control group had significant differences.The maximum apoptosis rate was 60.07%.Conclusion：The new monomer 3 inhibited human gastric cancer SGC-7901 cells significantly and had the impact on cell cycle distribution of the SGC-7901 cells and induction of apoptosis.

关 键 词：胃癌 温郁金 凋亡 

分 类 号：R285[医药卫生—中药学]