好氧条件下Sphingomonas sp.XJ1降解DBP途径的研究  

Study on degradation pathway of DBP by Sphingomonas sp. XJ1 under aerobic condition

作　　者：张新[1] 胡培磊[1] 周洪波[1,2] 

机构地区：[1]中南大学资源加工与生物工程学院,湖南长沙410083 [2]中南大学生物冶金教育部重点实验室,湖南长沙410083

出　　处：《现代生物医学进展》2010年第6期1110-1113,共4页Progress in Modern Biomedicine

基　　金：教育部新世纪优秀人才支持计划项目(NECT-06-0691);国家自然科学基金创新研究群体科学基金资助项目(50621063)

摘　　要：在三角瓶中采用Sphingomonas sp.XJ1对邻苯二甲酸丁酯(DBP)进行好氧降解,以考察DBP的降解途径。分别对降解16h、32h和40h的DBP样品进行代谢产物分析,可判定保留时间为4.79min和5.11min所对应的代谢产物分别为原儿茶酸和邻苯二甲酸。由此可知,菌株Sphingomonas sp.XJ1对DBP的降解遵循DBP好氧生物降解途径的一般途径。即在菌株XJ1的作用下,DBP首先水解为MBP,继而水解为PA,经由PCA最终完全降解为CO2和H2O。The aerobic degradation of DBP by Sphingomonas sp. XJ1 in flask was conducted to investigate the degradation pathway of this compound. The metabolites of DBP samples which had been degraded for 16h,32h,40h were analyzed respectively. The results suggested that the metabolites that had retention time of 4.79 min and 5.11 min were protocatechuic acid and phthalic acid respectively. It can be concluded that degradation of DBP by strain Sphingomonas sp. XJ1 followed the general pathway of aerobic biodegradation of this compound. It means that DBP was first hydrolyzed to MBP, and then was hydrolyzed to PA, it was ultimately degraded to CO2 and H2O via the intermediate of PCA.

关 键 词：DBP 生物降解 代谢途径 

分 类 号：Q331[生物学—遗传学]