不同温度下成骨细胞增殖及其OPG/RANKL mRNA表达的影响  

Mild heat shock’s effect on the proliferation and OPG/RANKL mRNA expression in Osteoblast

作　　者：甘璐[1] 吴进[1] 周勇[1] 杨彤涛[1] 

机构地区：[1]第四军医大学唐都医院骨科,陕西西安710038

出　　处：《现代生物医学进展》2010年第5期847-850,共4页Progress in Modern Biomedicine

基　　金：陕西省自然科学基金(2007C243)

摘　　要：目的:探讨不同温度下对小鼠成骨细胞MC3T3-E1的增殖以及OPG/RANKL表达水平的影响。方法:1.以小鼠成骨细胞MC3T3-E1为体外实验模型,MTT法检检测细胞的增殖情况。2.RT-PCR方法检测MC3T3-E1OPG/RANKL mRNA的表达水平。结果:设定对照组为37℃,高于对照组(38℃-39℃-40℃-41℃-42℃)分别作用于MC3T3-E1细胞1小时/天,连续1周,可刺激细胞增殖,OD值显著增加(P<0.05)。同时可增加OPG mRNA表达,降低RANKL mRNA表达,呈温度梯度依赖性。结论:热刺激促进MC3T3-E1细胞增殖,同时通过调节OPG/RANKL mRNA的表达,直接促进骨形成,抑制骨吸收。Objective：To study the effect of mild heat shock on proliferation and the expression of osteoprotegerin （OPG） and receptor activator of NFK B ligand （RANKL） mRNA in MC3T3-E 1 osteoblasts of mice.Methods：The osteoblasts MC3T3-E 1 were cultured in vitro,the effects of different degrees of miled heat on osteoblast proliferation were assessed by MTT colorimetry;the expression OPG and RANKL mRNA in MC3T3-E1 was analyzed by semi-quantative RT-PCR.Results：Proliferation of MC3T3-E1 was promoted by mild heat shock （37℃-38℃-39℃-40℃-41℃-42℃ ）after 1 h/d x lw,Semi-quantative RT-PCR examination revealed：Mild heat shock promote OPG mRNA expression and inhibit the RANKL mRNA expression, in temperature gradient-dependent manner.Conclusion： Mild heat shock promotes proliferation in MC3T3-E1 osteoblasts of mice,and promotes OPG mRNA expression and inhibits RANKL mRNA expression,accordingly promotes bone formation,and inhibits bone resorption.

关 键 词：RANKL OPG 成骨细胞 增殖 

分 类 号：Q95-3[生物学—动物学] R322.71[医药卫生—人体解剖和组织胚胎学]