叶用芥菜细胞悬浮培养的研究  

Study on Cell Suspension Culture for Leaf Mustard

作　　者：蒋波[1] 王志敏[1] 汤青林[1] 宋明[1] 宋波[1] 

机构地区：[1]西南大学园艺园林学院,重庆市蔬菜学重点实验室,重庆400716

出　　处：《西南大学学报（自然科学版）》2010年第4期70-74,共5页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市自然科学基金资助项目(2009BB1307)

摘　　要：以叶用芥菜的子叶为外植体,对愈伤组织诱导和细胞悬浮培养的技术体系进行了研究.结果表明,愈伤组织诱导的最适培养基为MS+0.3mg/LNAA+1.0mg/L6-BA+0.1mg/L2,4-D,愈伤组织最佳增殖培养基为MS+3.0mg/L6-BA+1.0mg/L2,4-D.悬浮培养过程中细胞生长曲线呈S型,培养2～10d达对数生长期.将小细胞团接种于MS+1.0mg/LNAA+3.0mg/L6-BA分化培养基上,继代两次后,分化出芽,分化芽在培养基MS+0.1mg/LNAA上诱导生根,形成小植株.The cotyledons of leaf mustard were taken as explants to study the techniques of callus induction and cell suspension culture.The results showed that the optimal medium for callus induction was MS＋0.3 mg/L NAA＋1.0 mg/L 6-BA＋0.1 mg/L 2,4-D,while the optimal formula for callus proliferation was MS＋3.0 mg/L 6-BA＋0.1 mg/L 2,4-D.Cell growth process in suspension culture exhibited an S-shaped curve and a logarithmic growth phase was reached after 2 to 10 days＇ culture.When the calli of suspension culture were transferred to an MS medium containing 1.0 mg/L NAA and 3.0 mg/L 6-BA and sub-cultured twice,shoots differentiated from the calli.Rooting plantlets were induced on MS with 0.1 mg/L NAA.

关 键 词：叶用芥菜 愈伤组织 悬浮培养 植株再生 

分 类 号：Q949.748.3[生物学—植物学]