银杏叶提取物对颅脑损伤后脑血管内皮表达TM和vMF的影响及意义  

Effect of extract of Ginkgo biloba on expressions of TM and vMF in cerebrovascular endothelial cells early following diffuse traumatic brain injury in rats

作　　者：黄胜明[1] 马辉[2] 钱志远[1] 王爱东[1] 

机构地区：[1]苏州大学附属第二医院神经外科,215004 [2]济宁市第一人民医院神经外科,272000

出　　处：《中国临床神经外科杂志》2010年第4期231-234,共4页Chinese Journal of Clinical Neurosurgery

摘　　要：目的探讨早期应用银杏叶提取物(EGB)对大鼠颅脑损伤后脑血管内皮细胞的保护和调节作用。方法将140只大鼠按随机数字表法随机分为对照组和EGB治疗组,按Marmarous等人的方法建立弥漫性脑损伤模型,EGB治疗组在伤后即刻腹腔注射EGB,以后每24h腹腔注射一次直至被处死,对照组注射等量生理盐水。分别在伤后1h、4h、8h、12h、24h、3d、7d七个时间点断头取脑组织,采用免疫组化和荧光定量PCR测定大脑皮层脑血管内皮细胞不同时间点血栓调节蛋白(TM)和假性血管性血友病因子(vWF)蛋白表达水平,并观察大鼠皮层血管变化及皮层病理学变化。结果伤后4h至伤后3dEGB治疗组脑组织中TM和vWF的表达水平与对照组相比明显下降(P<0.05)。结论EGB可以抑制大鼠弥漫性颅脑损伤后脑血管内皮细胞活化标志物TM和vWF的表达,其机制可能与EGB抑制脑血管内皮细胞的活化有关。Objective To investigate the effect of extract of Ginkgo biloba（EGB）on cerebrovascular endothelial cell earlyf ollowing diffuse traumatic brain injury（TBI）.Method TBI models were established by Marmarous method in 140 rats,who werer andomly divided into control and treatment groups.In the treatment group,each rat received an intraperitoneally injection of EGBi mmediately after the injury and then every 24 h until the rat was executed.The expressions of Thrombomodulin（TM）,von Willebrandf actor（vWF）and their mRNA were determined respectively by immunohistochemical techniques and real-time quantitative reverset ranscription polymerase chain reaction（RT-PCR）1,4,8,12,24,72 and 168 hours after TBI in both the groups.Results The expressionl evels of TM,vWF and their mRNA were significantly lower in the treatment group than those in the control group 4,8,12,24 and 72h ours after TBI.Conclusion It is suggested that EGB may inhibit cerebrovascular endothelial cells activation by inhibition of thee xpression levels of the cerebrovascular endothelial cells activation markers,TM and vWF following diffuse TBI in rats.

关 键 词：颅脑损伤 脑血管内皮细胞 血栓调节蛋白 假性血管性血友病因子 银杏叶提取物 大鼠 

分 类 号：R651.15[医药卫生—外科学] Q786[医药卫生—临床医学]