不同晶相草酸钙超细微晶的合成及其与硫酸软骨素A的相互作用  

Preparation of Ultrafine COM and COD Crystallites and Their Interaction with Chondroitin Sulfate A

作　　者：谢蓉[1] 杨锦[1] 万牡华[1] 欧阳健明[1] 

机构地区：[1]暨南大学生物矿化与结石病防治研究所,广州510632

出　　处：《无机化学学报》2010年第4期560-566,共7页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金(No.30672103);广东省科技攻关项目(No.2009B030801236)资助

摘　　要：采用配位沉淀法制备了二水草酸钙(COD)和一水草酸钙(COM)超细微晶,其尺寸分别为150nm和320nm。通过扫描电子显微镜(SEM)、X射线衍射(XRD)、红外光谱(FTIR)、纳米粒度仪(Nano-ZS)和紫外-可见吸收光谱(UV-Vis)表征了这两种草酸钙微晶。研究了尿大分子硫酸软骨素A(C4S)对上述COM和COD微晶ξ电位、粒径、聚集程度和紫外吸光度的影响。随着cC4S从0增加到1.0g·L-1,COD微晶的ξ电位从-9.7mV减小到-46.1mV,COM微晶的ξ电位从-15.9mV减小到-49.0mV;微晶表面ξ电位变负后,有利于稳定溶液中悬浮的微晶。在水溶液中,COD和COM微晶均存在显著的聚集现象,而C4S的存在可抑制COD微晶的聚集,并在浓度为0.05g·L-1时抑制效果最好。由于尿液中存在大量草酸钙微晶,本研究有助于阐明草酸钙结石的形成机理和C4S对草酸钙结石形成的抑制作用。Ultrafine crystallites of calcium oxalate dihydrate （COD） and monohydrate （COM）, with a size of 150 nm and 320 nm respectively, were prepared by complexing precipitation method. Scanning electron microscopy （SEM）, X-ray diffractometer （XRD）, FTIR, nano-particle size analyzer （Nano-ZS） and ultra-visible （UV-Vis） spectrum were used to characterize these two ultrafine crystallites. The effect of urinary macromolecule of chondroitin sulfate A （C4S） on the properties of ultrafine COM and COD crystallites was investigated. These properties included ξ potential value, particle size, aggregation and ultraviolet absorbance. When the concentration of C4S increased from 0 to 1.0 g· L-1, the ξ potential of ultrafine COD crystallites decreased from -9.7 mV to -46.1 mV, and that of ultrafine COM crystallites decreased from -15.9 mV to -49.0 mV. A much more negative ξ potential value is helpful for the stabilization of crystallites suspended in solution. Both COM crystallites and COD crystallites markedly aggregated in the water, but the presence of C4S can inhibit the aggregation of crystallites, and the best concentration of C4S for inhibition was 0.05 g·L^-1 Since there are a great deal of calcium oxalate crystallites in urine, the experimental results in this work may help to clarify the formation mechanism of calcium oxalate stones and the inhibition of C4S on formation of calcium oxalate stone.

关 键 词：超细微晶 草酸钙 硫酸软骨素A 生物矿化 

分 类 号：O614.231[理学—无机化学]