不同连接比例叶酸偶联壳聚糖的制备  

Preparation of chitosan coupled with folate by different connection ratio

作　　者：赵颖[1] 杨琰[2] 卢实[2] 张红菱[1] 

机构地区：[1]武汉工业学院生物与制药工程系,湖北武汉430023 [2]华中科技大学同济医学院协和医院,湖北武汉430030

出　　处：《西安交通大学学报（医学版）》2010年第3期385-387,共3页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的叶酸与壳聚糖偶联,并制备不同连接比例的叶酸偶联壳聚糖。方法通过叶酸活性酯与壳聚糖上的氨基反应,制得叶酸偶联壳聚糖;采用红外光谱法验证偶联是否成功,紫外分光光度法测定每个壳聚糖上连接叶酸的量。结果叶酸偶联壳聚糖红外光谱图表明,叶酸与壳聚糖成功偶联,并制备了每个壳聚糖上连接叶酸的量大致为3个、10个、20个、30个的叶酸偶联壳聚糖。结论叶酸与壳聚糖成功偶联,并成功制备了不同连接比例的叶酸偶联壳聚糖。Objective To couple folate with chitosan and prepare folate-coupled chitosan by different connection ratio.Methods The folate-coupled chitosan was prepared by the reaction of the activated folate ester with the amine group on the chitosan.Then the folate-coupled chitosan was verified by infrared spectroscopy.The number of folates in each of the chitosans was measured by UV spectrophotometry.Results Infrared spectroscopy showed that the folate-coupled chitosan was prepared successfully,and the number of folates in each chitosan was approximately 3,10,20 and 30.Conclusion Coupling of folate with chitosan was successful,and folate-coupled chitosan by different connection ratio was successfully prepared.

关 键 词：叶酸 壳聚糖 偶联 基因治疗 

分 类 号：Q819[生物学—生物工程]