竹横锥大象对寄主及虫体挥发物的行为和触角电位反应  

Behavioral and EAG responses of Cyrtotrachelus buqueti Guerin-Meneville (Coleoptera:Curculionidae) adults to host volatiles and their body extracts

作　　者：杨桦[1,2] 杨茂发[1] 杨伟[2] 杨春平[2] 朱天辉[2] 黄琼[2] 赵晓英[2] 

机构地区：[1]贵州大学昆虫研究所,贵阳550025 [2]四川农业大学森林保护重点实验室,四川雅安625014

出　　处：《昆虫学报》2010年第3期286-292,共7页Acta Entomologica Sinica

基　　金："211工程"双支计划资助项目;长江上游生态林业工程建设资助项目

摘　　要：为了探明竹横锥大象Cyrtotrachelus buqueti Guerin-Meneville是否存在两性间的引诱作用,以及寄主植物慈竹Neosinocalamus affinis笋对两性行为的影响,采用Y型嗅觉仪测定了竹横锥大象雌、雄成虫对4种虫体及寄主挥发物不同处理(雌、雄成虫,慈竹笋与雌、雄成虫的复合体)的行为反应,测定了触角不同部位对雌雄成虫3种虫体的提取物(整体、鞘翅、后肠)及其与寄主植物挥发物质联合作用的触角电位反应(EAG)。结果表明:雌成虫挥发物对雄虫有较高的引诱活性,对雌虫有驱避作用;雄成虫挥发物对雌虫有一定的引诱作用。雌、雄成虫对虫体与竹笋复合体气味的行为和电生理反应均显著或极显著高于对相应虫体挥发物的反应。经EAG测定,触角各部位对虫体不同部位挥发物的EAG值差异明显,触角端部对虫体与植物挥发性物质的联合作用EAG反应差异极其显著(P<0.01)。竹横锥大象对沾染雌雄成虫虫体提取物的玻璃棒有一定的兴奋表现,雌虫兴奋率为6.67%,雄虫为26.67%。结果提示,竹横锥大象种内存在信息素,雌虫释放的信息素对同类雄虫有很强的引诱作用,而雄虫所释放的信息素对同类雌虫有引诱作用,添加寄主植物能够增强两性间的引诱效果。To find whether there is attraction between the male and female of Cyrtotrachelus buqueti Guerin-Meneville and the influence of the host plant on the behavior of the male and female,Y-tube olfactometer was used to determine the behavioral reaction of adult female and male to the volatiles of their bodies and the host plant after different treatments,and the EAG responses of different portions of antenna of C.buqueti to different combinations of their bodies extracts （female and male） and the volatiles of the host plant.The results showed that the volatiles of the female adult had higher luring activity to the male adult but had repelling action to the female adult,and that of the male adult had certain luring activity to the female adult.The behavioral and electrophysiological responses of female and male adults to the combined odour from their bodies and bamboo shoots were obviously stronger than those to the corresponding volatiles of their bodies only.The EAG response tests showed that the EAG values of different antennal parts to the volatiles from different body parts of C.buqueti adults were obviously different,and the EAG responses of the terminal portion of the antenna to the different combinations of beetle body extracts and the volatiles of the host plant were significantly different （P0.01）.Adults showed the excitement to the extracts of the adult male or female bodies when touched by a glass rod dipped with the extracts,and the excitement rate of female was 6.67%,while that of the male was 26.67%.The results suggest that the pheromones exist in C.buqueti.The pheromones from the female has great allure to the male of the same species and that from the male also has allure to the female of the same species,while the host plant could strengthen the allure between the female and the male.

关 键 词：竹横锥大象 慈竹 植物挥发物 虫体挥发物 触角电位反应 行为反应 Y型嗅觉仪 

分 类 号：Q968[生物学—昆虫学]