人类胚胎干细胞分化过程中DPPA2基因的表达情况分析(英文)  

The Expression Pattern of DPPA2 during Differentiation of Human Embryonic Stem Cells

作　　者：陈天姬[1] 杜娟[1] 杜丽丽[1] 欧阳琦[1] 卢光琇[1] 

机构地区：[1]中南大学生殖与干细胞工程研究所,中国湖南长沙410078

出　　处：《生命科学研究》2010年第2期106-111,共6页Life Science Research

基　　金：国家自然科学基金资助项目(30570937);国家高技术研究发展计划项目(2006AA02A102);中南大学研究生创新基金资助项目(2340-74334000003)~~

摘　　要：DPPA2(Developmental Pluripotency-Associated gene2)是近年来发现的在多能性细胞中特异表达的一个基因,它被认为参与维持干细胞的"干性".但目前为止,并没有关于该基因在人类胚胎干细胞(human embryonic stem cells,hESCs)分化过程中的表达情况的报道,其功能也尚不清楚.通过Real-time PCR对DPPA2基因在hESCs分化过程中的表达情况进行分析,此外还对其在异常核型hESCs,人类胚胎癌细胞(human embryonic carcinoma cells,hECCs)NTERA-2以及其它5种癌细胞中的表达情况进行检测.结果表明DPPA2基因在hESCs中特异表达,其表达水平随着hESCs的分化而显著下调.该基因在异常核型hESCs和NTERA-2细胞中也有表达,但在其它肿瘤细胞中未检测到该基因的表达.此外,以EGFP-N1系统为基础的亚细胞信号定位结果表明,DPPA2是一个核蛋白.这些结果提示,DPPA2基因可能在维持hESCs特性的过程中发挥着重要的作用.DPPA2 （Development Pluripotency-Associated Gene 2） has been identified recently as a gene that is specially expressed in pluripotent cells and considered involving in preservation of sternness status. But the expression file and function of DPPA 2 in human embryonic stem cells （hESCs） have not yet been reported. Here, the expression pattern of DPPA2 was analyzed during hESCs differentiation using real-time PCR. The expression of DPPA2 in abnormal karyotype hESCs, human embryonic carcinoma cells （hECCs,NTREA-2） and other 5 tumor cell lines was also detected. The results showed that DPPA 2 was specifically expressed in hESCs and its mRNA level decreased markedly during hESCs differentiation. DPPA2 was also expressed in abnormal karyotype hESCs and NTREA-2 cells but other tumor ceils. Furthermore, the result of subcellular localization using EGFP-N1 （Enhanced Green Fluorescent Protein） system demonstrated that DPPA2 was a nuclear protein. These data indicated that DPPA2 might be a potential element of the molecular machinery governing crucial properties of hESCs.

关 键 词：DPPA2基因 人类胚胎干细胞 干性 核蛋白 

分 类 号：Q291[生物学—细胞生物学]