LMO3过表达对胶质细胞增殖的作用  

Overexpression of LMO3 Increases Proliferation of Glia Cells

作　　者：惠玲[1] 吕同德[1] 王建峰[1] 王晓辉[1] 哈小琴[1] 张军莉[1] 杨桂兰[2] 鞠英[2] 

机构地区：[1]兰州军区兰州总医院医学实验中心甘肃省干细胞与基因药物重点实验室,中国甘肃兰州730050 [2]兰州军区兰州总医院皮肤科,中国甘肃兰州730050

出　　处：《生命科学研究》2010年第2期112-116,共5页Life Science Research

基　　金：国家自然科学基金资助项目(30400125);中国博士后科学基金资助项目(20070410230)

摘　　要：构建重组质粒pcDNA4/HisA-LMO3,转染C8-D1A胶质细胞,对照组为pcDNA4/HisA空载体质粒转染组和未转染组,MTT观察各组细胞的体外生长情况,流式细胞术(FCM)测定各组细胞的细胞周期和凋亡细胞百分比,Western-blot检测LMO3转染后凋亡相关蛋白的表达变化,从而观察LMO3基因对C8-D1A胶质细胞体外生长的影响.RT-PCR、Western印迹显示pcDNA4/HisA-LMO3转染组LMO3mRNA及LMO3蛋白表达水平明显高于对照组;与对照组细胞相比,转染组细胞的增殖能力明显高于空载体对照组及C8细胞(P<0.01),S期细胞增加,G0/G1期细胞减少,C8细胞转染LMO3后可以促进C8细胞从G0/G1期进入S期,从而促进细胞的增殖.C8 glia cells were cultured in DMEM with 10% FBS. Recombinant plasmid pcDNA4/HisA- LMO3 was constructed and transfected into the C8 cells with the lipofeetamine 2000. A stable C8 cell line overexpressing LMO3 was established to observe the direct effect of LMO3 on C8 cells. Parental C8 cells and parental C8 cells stably transfected with blank vector pcDNA4/HisA were used as control groups. The cell growth was determined by MTT method and flow cytometry （FCM） was used to analyze the cell cycle in each group. The stably transfected cell line C8-LMO3 was confirmed by RT-PCR and Western blotting. After transfection with LMO3, C8 cells showed significant increase in cell proliferation when compared with the control groups. The percentage of S phase cells increased, whereas G0/G1 phase cells decreased. Overexpression of LMO3 can stimulate prroliferation by promoting glia cell into cell cycle.

关 键 词：LMO3 基因转染 胶质细胞 增殖 

分 类 号：Q74[生物学—分子生物学]