响应面法优化延胡索生物碱乙醇提取工艺研究  

Optimization of Extraction of Alkaloids from Corydalis yanhusuo W.T. Wang by Response Surface Methodology

作　　者：商小金[1] 钱俊青[1] 郭辉[1] 

机构地区：[1]浙江工业大学药学院,浙江杭州310014

出　　处：《林产化学与工业》2010年第2期32-36,共5页Chemistry and Industry of Forest Products

基　　金：浙江省教育厅科技项目(20060808)

摘　　要：为了提高中药延胡索的利用率,采用响应面法优化其生物碱的提取工艺。考察了不同反应条件对延胡索生物碱得率以及生物碱纯度的影响。在单因素试验基础上,以生物碱得率为指标,通过响应面法对提取工艺条件进行优化。得到的优化条件为:提取时间116 min,提取温度49℃,乙醇体积分数80%,乙醇用量10 mL/g(以延胡索计)。在此条件下提取3次,生物碱得率为0.598%,其纯度为17.38%。For improving the yield of alkaloids from Corydalis yanhusuo W. T. Wang, response surface methodology was adopted to optimize the extraction process. The effects of different parameters on yield and purity of alkaloid were studied. Based on response surface methodology, the extraction conditions on the basis of single factor experiment using alkaloid yield as index were optimized. The optimal conditions were： extraction time 116 min, extraction temperature 49℃, 80 % （volume fraction） EtOH as solvent, EtOH dose 10 mL/g （ based on raw material）. Under optimal conditions, the yield of alkaloids and purity were 0.598 % and 17.38 % respectively, after three times extraction.

关 键 词：延胡索 生物碱 响应面法 提取 

分 类 号：TQ351[化学工程]