QCM-D研究蛋白质与多糖的相互作用  

Interactions between Protein and Polysaccharide Studied by QCM-D

作　　者：林园[1] 黄庆荣[2] 苏朝晖[1] 

机构地区：[1]中国科学院长春应用化学研究所,高分子化学与物理国家重点实验室,长春130022 [2]Rutgers大学食品科学系,美国新泽西州08901

出　　处：《应用化学》2010年第5期505-509,共5页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金(20774097,20423003);基金委创新群体(50621302)资助项目

摘　　要：应用具有耗散因子功能的石英晶体微天平(QCM-D)检测了多糖多层膜的构筑过程以及蛋白质在多层膜上的吸附行为。研究结果表明,通过层层组装的方法,可以实现多糖卡拉胶多层膜的构筑,并且随着卡拉胶电荷密度的增加,其组装量减少。研究了牛体血清蛋白(BSA)在卡拉胶上的吸附行为。结果发现,BSA的吸附行为强烈地依赖于卡拉胶的电荷密度和基体的粗糙度。通过QCM-D实时监测了BSA在卡拉胶上的动力学吸附曲线,同时根据ΔD值推测了BSA在不同的基底表面上所形成的结构及吸附行为。Polysaccharide surfaces were constructed from carrageenan and poly（diallyldimethylammonium chloride）（PDDA） via layer-by-layer assembly technique.The adsorption of bovine serum albumin（BSA）,an ellipsoidal model protein,on the surface of the multilayers was studied in detail by means of a quartz crystal microbalance with dissipation monitoring（QCM-D）.Four carrageenans with different charge densities were investigated,and the amount of the carrageenan assembled was found to decrease with the increase of the charge density.When BSA was adsorbed on the PDDA surface,the dissipation factor（ΔD） was small,indicating that BSA lay down on the surface to form a thin layer.When BSA was adsorbed to the ι-carrageenan（IC） surface,both the QCM frequency shifts（ΔF） and the dissipation factor ΔD were larger,suggesting that BSA stood on the carrageenan surface to form a thicker layer.In addition,it was found that both the amount of adsorption and the packing of BSA on the carrageenan surface depended on the charge density and the surface roughness of the carrageenan substrate.

关 键 词：石英晶体微天平(QCM-D) 蛋白质 多糖 

分 类 号：O636.1[理学—高分子化学]