热处理的锐钛型二氧化钛催化可见光照射损伤牛血清白蛋白  

Damage of Bovine Serum Albumin Catalyzed by Heat-Treated Anatase Titanium Dioxide under Visible Light Irradiation

作　　者：张朝红[1] 韩文明[1] 王冬梅 胥丹平[1] 邓英俏[1] 陈中林[1] 郜炜[1] 

机构地区：[1]辽宁大学环境学院,沈阳110036 [2]锦州市环境监测中心站综合技术室

出　　处：《应用化学》2010年第5期594-599,共6页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金(20371023);辽宁省教育厅自然科学基金(05L154)资助项目

摘　　要：利用紫外-可见(UV-Vis)光谱和荧光光谱研究了可见光照射热处理的锐钛型二氧化钛(TiO2)对牛血清白蛋白(BSA)的损伤,同时对处理前后TiO2的光催化活性进行了比较,并考察了可见光照射时间、TiO2加入量和照射功率等因素对BSA分子损伤的影响。结果表明,经过高温处理锐钛型TiO2可以部分转晶,处理后的TiO2和可见光的联合作用能够导致BSA分子损伤。UV-Vis光谱表现为增色效应,荧光光谱表现为荧光猝灭。且处理后的TiO2的光催化活性好于处理前。对于体系温度为25℃和浓度为1.0×10-5mol/L的BSA溶液,BSA分子的损伤程度随着可见光照射时间、TiO2加入量和照射功率的增加而增大。同步荧光光谱证明了BSA分子中酪氨酸的损伤程度较大。In order to efficiently make use of visible light and enhance photocatalytic activity,the rutile crystal TiO_2 catalysts which could utilize visible light were obtained by heat-treated method.The damage of BSA under the visible light irradiation in the presence of heat-treated anatase TiO_2 was investigated by means of UV-Vis and fluorescence spectroscopies.The photocatalytic activity of the heat-treated TiO_2 was compared with that of the untreated one.In addition,the influences of irradiation time,TiO_2 addition dose and irradiation power on the damage of BSA molecule were studied.The results show that the damage of BSA under the visible light irradiation was achieved in the presence of heat-treated anatase TiO_2.The photocatalytic activity of heat-treated TiO_2 was also better than that of the untreated one.The obvious hyperchromic effect in UV-Vis spectra and the fluorescence quenching in fluorescence spectra were both observed,which resulted from the exposure of aromatic heterocycle residues of tryptophane and tyrosine.Moreover,the damage degree of tyrosine residues was greater than tryptophan residues,which was proved with synchronous fluorescence spectra.For a BSA solution of 1.0×10-5 mol/L at 25 ℃,the damage of BSA was aggravated with the increase of the irradiation time,TiO_2 addition dose and the irradiation power.They are of significance to the study on the application of scavenger and inactivator for some virus.

关 键 词：可见光 热处理 二氧化钛(TiO2) 损伤 牛血清白蛋白(BSA) 

分 类 号：O644.3[理学—物理化学]