利用同工酶、RAPD技术及形态学标记对不同地域木豆品种的遗传多样性研究  

Research of Genetic Diversity on Pigeonpea Collected in Different Areas Using Methods of Isozyme,RAPD Maker,Morphology

作　　者：蒋慧萍[1] 李杨瑞[2] 

机构地区：[1]广西农业科学院生物技术研究所,广西南宁530007 [2]广西农业科学院广西作物遗传改良生物技术重点开放实验室,广西南宁530007

出　　处：《西南农业学报》2010年第2期483-486,共4页Southwest China Journal of Agricultural Sciences

基　　金：广西农科院院科技发展基金项目(2002003)

摘　　要：试验选用5个不同地理来源的木豆品种(分别来自广西、海南、非洲、缅甸和印度)和3个ICR ISAT杂交木豆品种(ICPH8、ICPL87091、ICPL87119)作为供试材料。分别采用形态标记、过氧化物酶同工酶标记和RAPD标记对8个品种资源的遗传多样性进行研究,并对形态标记及RAPD试验结果进行了聚类分析,结果表明:①通过对过氧化物酶同工酶酶谱分析,发现8个木豆品种的电泳图谱谱带条数和迁移距离相同,说明8个木豆品种遗传相似性高;②通过对8个品种1年的表型性状观察,发现各品种在表型上存在一定差异,如生育期、花色和粒色等。将表型的18个数量性状进行聚类分析,发现各品种间的遗传相似系数高;同时对8个品种进行RAPD标记分析,并绘制亲缘关系系谱图,同样也得到各品种间的遗传距离很近,平均为0.25。从而证实了8个木豆品种遗传基础接近,具有较近的亲缘关系。Five different pigeonpea varieties from different areas （i. e. Guangxi, Hainan, Africa, Burma and India ） and three hynrids bred by ICRISAT were investigated. The biological characters were investigated in the field. And the biological morphological markers, peroxidase isozymes and RAPD techniques were used for clustering the varieties. The results showed as follows： （i） The result of peroxidase isozyme showed that the numbers of electrephoresis profile and the moving distance were the same in 8 different varieties. （ii） Base on the observation on the 8 pigeonpea varieties for one year, some differences were detected among these varieties, such as growth duration, flower color and seed color etc. The results of cluster analysis in 18 quantity characters demonstrated that there was a high proximity between varieties. The cluster analysis of RAPD data showed the similar results, and the average genetic distance was 0.25. It further proved that they had a narrow genetic basis, and showed a close genetic relationship.

关 键 词：木豆 遗传多样性 过氧化物酶同工酶 表型性状 RAPD标记 聚类分析 

分 类 号：S643.9[农业科学—蔬菜学]