香叶天竺葵离体芽的培养及生产应用研究  

Study on in vitro Shoot Culture of Pelargonium graveolens and Its Production

作　　者：易清元[1] 夏凯国[1] 江明[1] 任洪涛[1] 

机构地区：[1]云南农业大学香料研究所,云南昆明650051

出　　处：《西南农业学报》2010年第2期561-564,共4页Southwest China Journal of Agricultural Sciences

基　　金：云南省自然科学基金项目(2008ZC054M)

摘　　要：香叶天竺葵生产上采用扦插繁殖，存在病害加重，品种退化的现象。本实验用离体芽进行诱导培养，探索产业化快繁优质种苗的方法，从而达到改良品种的目的。试验表明：MS＋BA0．5～2．0mg／L＋NAA0～0．2mg／L的配比可直接诱导产生较多的不定芽；MS＋NAA0．1mg／L可作为微型扦插的继代培养基；MS＋IBA0．5mg／L为最佳生根培养基，试管苗移栽成活率可达95％。In Pelargonium graveolens plant production,reproduction using cottage could cause cultivar degeneration and more diseases. For improving cultivar characteristics and promoting industrial plant production, in vitro shoot culture was carried out in this study. The results showed that combinations of MS ＋ BA 0.5 - 2.0 mg/L ＋ NAA 0 - 0.2 mg/L could induce more adventitious buds, MS ＋ NAA 0.1 mg/L was used as subculture medium for micro cottages, and MS ＋ IBA 0.5 mg/L was used as the best rooting medium with 95 % survival rate of Pelargonium graveolens in vitro plants.

关 键 词：香叶天竺葵 离体芽 组织培养 快速繁殖 

分 类 号：S796[农业科学—林木遗传育种]