甘蔗二点螟杀虫真菌绿僵菌的筛选与毒力测定  

Screening and Virulence Test of Metarhizium Strains Against Chilotraea influscatellus (Snellen)

作　　者：詹儒林[1] 常金梅[1] 何衍彪[1] 

机构地区：[1]中国热带农业科学院南亚热带作物研究所,广东湛江524091

出　　处：《热带作物学报》2010年第1期111-115,共5页Chinese Journal of Tropical Crops

基　　金：国家支撑计划(2007BAD48B02)

摘　　要：从椰心叶甲僵虫上分离得到10株对甘蔗二点螟具有较好致病性的绿僵菌,其中MC3的致病性最好,校正死亡率为89.16%,且甘蔗二点螟的校正死亡率随着MC3菌株孢子浓度的增加而逐渐提高,当浓度为1.0×107个/mL时,其死亡率为93.02%。MC3菌株在SDAY培养基菌丝生长及产孢效果最好,孢子萌发的最适温度是25～28℃。Twelve Metarhizium strains were isolated from dead bodies of coconut leaf beetles （Brontispa longissina （Gestro））. Among the strains, 10 showed higher pathogenicity against Chilotraea influscatellus （Snellen）, an insect pest, than the control, of which the strain MC3 was found most efficient in controlling of this insect pest. The corrected mortality rate of C. influscatellus inoculated with the strain MC3 was increased gradually with the spore concentration of Metarhizium. About 93.02% of the adults of the insect pest were controlled with the strain MC3 at a spore concentration of 1.0×107 conidia/mL. The strain MC3 showed the best mycelial growth and sporulation on the SDAY, and the optimum temperature for the spore germination was 25～28 ℃.

关 键 词：二点螟 绿僵菌 致病性 

分 类 号：S566.1[农业科学—作物学] S476