高效液相色谱法测定礞石滚痰片中黄芩苷含量  

Determination of Baicalin Contents in Mengshi Guntan Tablets By HPLC

作　　者：李卿[1] 陈志新[2] 秦剑[1] 肖铭玉[1] 

机构地区：[1]重庆市中药研究院,重庆400065 [2]中国人民解放军第6905工厂职工医院,重庆400712

出　　处：《中国药业》2010年第8期38-38,共1页China Pharmaceuticals

摘　　要：目的测定礞石滚痰片中黄芩苷的含量。方法采用高效液相色谱(HPLC)法。色谱柱为DiamonsilTM(钻石)C18柱(250mm×4.6mm,5μm),以甲醇-0.2%磷酸溶液(47:53)为流动相,检测波长为280nm。结果黄芩苷进样量在0.184～2.760μg范围内与峰面积线性关系良好,平均加样回收率为98.52%,RSD为0.47%(n=6)。结论 HPLC法快速、准确,可用于该制剂的质量控制。Objective To determine the contents of baicalin in Mengshi Guntan Tablets. Methods The HPLC method was adopted and the DiamonsilTM C18 column （250 mm× 4.6mm, 5μm） was used. The mobile phase was methanol - 0. 2% phosphoric acid（47 ： 53）. The detection wavelength was at 280 nm. Results The calibration curve of baicalin was linear in the range of 0. 184 - 2. 760 μg, the average recovery rate was 98. 52%, RSD =0. 47% （n = 6）. Conclusion HPLC is quick and accurate, which can be used for the quality control of Mengshi Guntan Tablet.

关 键 词：黄芩苷 礞石滚痰片 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]