哮喘患者检测痰中基质金属蛋白酶的临床意义  

Clinical significance of test of metalloproteinase in sputum from asthma patients

作　　者：肖可为[1] 辛晓峰[2] 刘雪梅 何宏伟[1] 

机构地区：[1]南京军区联勤部门诊部,江苏南京210002 [2]南京军区南京总医院呼吸科,江苏南京210002 [3]南京建邺区医院检验科,江苏南京210000

出　　处：《东南国防医药》2010年第2期112-114,共3页Military Medical Journal of Southeast China

摘　　要：目的探讨支气管哮喘(简称哮喘)患者诱导痰中基质金属蛋白酶9(MMP-9)和基质金属蛋白酶抑制剂1(TIMP-1)的浓度比值与气道炎症和气流受限的关系。方法选择缓解期哮喘患者(哮喘组)和健康对照者(健康对照组)进行肺功能测定;用诱导痰检查方法对痰炎性细胞进行分类计数,并用酶联免疫吸附试验(ELISA)法测定诱导痰上清液中MMP-9和TIMP-1浓度。结果哮喘组和健康对照组诱导痰中MMP-9、TIMP-1浓度比较差异和MMP-9/TIMP-1比值比较差异有统计学意义。结论哮喘组患者诱导痰中MMP-9/TIMP-1比值的失衡与气道炎症有关,从而造成气道重塑和气流受限,导致肺功能下降。Objective To investigate the relationship between the levels of metalloproteinase-9 （MMP-9） and tissue inhibitor of metalloproteinase-1 （TIMP-1） in induced sputum in patients with asthma and the airway inflammation and airflow limitation. Methods Sixteen patients with asthma in remission sta- ges, and twelve normal control subjects were included in this study. Lung function was measured. Induced sputum was obtained and processed for cell differential count. The concentrations of MMP-9 and TIMP-1 in supernatant were assayed by enzyme-linked immunosorbent assay （ELISA）. Results The difference of the concentration of MMP-9/TIMP-1 in induced sputum and the difference of the ratio of MMP-9/TIMP-1 in asthmatics and in normal control subjects were statistical analyzed. Conclusion The imbalance between MMP-9 and TIMP-1 in induced sputum in asthmatics is associated with airway inflammation and airflow llml- tation, which may play a role in the pathogenesis of extracellular matrix remodeling and airflow limitation.

关 键 词：哮喘 基质金属蛋白酶9 基质金属蛋白酶抑制剂1 气道炎症 气流受限 

分 类 号：R256.12[医药卫生—中医内科学]