野木瓜注射液对体外缺血清缺氧培养的小鼠脊髓神经细胞生长的影响  

Effects of injection stauntoniae on the growth of mouse spinal neurons under aerum free and anoxia condition in vitro

作　　者：张秀璋[1] 叶文博[1] 周丽娜[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《上海师范大学学报（自然科学版）》2010年第2期189-193,共5页Journal of Shanghai Normal University(Natural Sciences)

基　　金：上海师范大学科研项目(SK200715)

摘　　要：分离小鼠脊髓神经细胞进行原代培养，通过缺血清缺氧培养建立神经细胞损伤模型．受损的神经细胞中加入野木瓜注射液，利用MTT法测定神经细胞的活性，用倒置相差显微镜进行显微观察和测量．结果表明：浓度较高500mg／L时，野木瓜注射液对细胞有伤害作用，在50～300mg／L浓度范围内，能提高缺血清缺氧神经细胞的细胞活性，使细胞数和突起数增加，胞体直径增长，浓度为100mg／L时效果最明显．The spinal neurons were dissociated from primary mouse and cultured in vitro. The model of apoptosis induced by anoxia and aerum free was established on cultured neurons. After given Injection Stauntoniae （IS） 4 days, these cells were observed under inverted phase contrast microscope and tested MTT assay. The results indicated that IS caused cell injury at high concentrations （ 〉 500 mg/L）. However, IS could enhance the cytoactivity and increase the number of neurons and neurites, and the size of cell body at concentrations from 50 to 300 mg/L,especially at 100 mg/L.

关 键 词：小鼠 野木瓜注射液 脊髓神经细胞 缺血清缺氧培养 

分 类 号：Q954.6[生物学—动物学] R931.7[医药卫生—生药学]