不同种群狭叶小羽藓(Haplocladium angustifolium)重金属含量及遗传多样性  

Genetic diversity and heavy metal contents of different populations of the moss Haplocladium angustifolium

作　　者：李倩影[1] 曹同[1] 于晶[1] 俞鹰浩[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《上海师范大学学报（自然科学版）》2010年第2期194-199,共6页Journal of Shanghai Normal University(Natural Sciences)

基　　金：国家自然科学基金(30770141);上海市科委创新项目(08390513800);上海市教委重点项目(07ZZ61)

摘　　要：以上海市狭叶小羽藓居群为实验材料,测定不同居群植物体中的重金属元素含量,利用随机扩增多态DNA标记技术(random amplified polymorphic DNA,RAPD)对不同浓度重金属污染下的小羽藓居群的遗传多样性和遗传分化进行了初步分析.结果表明:104个检测位点中发现94个多态性位点,多态位点百分率为91.35%,总的居群的Nei基因多样性指数(H)为0.2682,Shannon指数(I)为0.4108,小羽藓种群表现出较高的遗传多样性.总的遗传居群间遗传分化系数(Gst)为0.5591,居群内遗传分化系数(Hs)为0.1182,居群间的变异程度远远大于居群内的变异程度.将各个样点居群的Shannon信息指数同重金属含量做相关性分析得出,Shannon信息指数的增加同Cr含量呈明显正相关,表明Cr胁迫对小羽藓种群的遗传分化有较大程度的影响.The plant samples of different populations of the moss Haplocladium angustifolium were collected from nine parks in Shanghai. Contents of heavy metals （Cd, Cr, Cu, Pb,Zn ） in the samples were determined, using random amplified polymorphic DNA markers（ RAPD）, genetic diversity and genetic differentiations of the different populations were analyzed. Among 104 test loci ,94 polymorphic loci were found,accounting to 91.35% of the total test loci. The total populations of the Haplocladium angustifolium ,with the Nei gene diversity index （H） of 0. 2682, Shannon index （I） as 0.4108, showed high genetic diversity. The genetic differentiation coefficient among populations （Gst） and within populations （Hs） are 0. 5591 and 0. 1182, respectively. It implied that the degree of variation among the different populations is far greater than the degree of variation within populations. Correlation analysis between contents of heavy metals and Shannon information index of the deferent populations showed that increase of the Shannon index was positively correlated to increase of the Cr content.

关 键 词：苔藓植物 狭叶小羽藓 重金属污染 遗传多样性 RAPD 

分 类 号：Q781[生物学—分子生物学]