石斛兰茎段组织培养再生体系的初步建立  

Primary Development of Tissue Culture Systems for Stem of Demdrobium nobile

作　　者：张彦妮[1] 钱灿[1] 陈立新 

机构地区：[1]东北林业大学,黑龙江哈尔滨150040 [2]黑龙江省农科院园艺分院,黑龙江哈尔滨150069

出　　处：《北方园艺》2010年第8期129-131,共3页Northern Horticulture

基　　金：黑龙江省博士后科研启动金资助项目(LBH-Q08156)

摘　　要：以石斛兰无菌苗茎段为外植体,通过在MS基本培养基中加入不同浓度的6-BA和NAA,筛选最佳培养基配方,从而初步建立石斛兰茎节的组织培养再生体系。结果表明:诱导茎节分化的最佳培养基是MS+6-BA 0.5 mg/L+NAA 0.1 mg/L,分化率为89.5%;最佳增殖培养基是MS+6-BA 2 mg/L+NAA 0.2 mg/L,增殖倍数为5.16;最佳生根培养基是1/2 MS+6-BA0.5 mg/L+NAA 0.1 mg/L,生根率为100%;移栽后成活率为97%。This paper took stems with nodes of tissue culture seedling as explants and screened out the best culture medium. Then regeneration system of Demdrobium nobile stem was established primarily. The results showed that the best culture medium for stems with nodes was MS supplemented with 0. 5 mg/L 6-BA and 0. 1 mg/L NAA, which the ratio of differentiation was 89.5%. The Best medium for proliferation was MS basal medium supplemented with 2 mg/L 6-BA and 0. 2 mg/L NAA. Proliferation multiple was 5.16. The best rooting medium was 1/2MS medium with 0. 5 mg/ L 6-BA and 0. 1 mg/L NAA. The rooting ratio was 100%. The survive ratio was 97% after transplanting.

关 键 词：石斛兰 茎节 组织培养 

分 类 号：S682.310[农业科学—观赏园艺]