拟南芥热激转录因子HSFA2基因克隆与原核表达  

Gene Cloning and Prokaryotic Expression of Heat Shock Transcription Factors HSFA2 from Arabidopsis thaliana

作　　者：王瑾瑜[1] 任亚萍[1] 

机构地区：[1]河南城建学院城市规划与建筑系,河南平顶460440

出　　处：《北方园艺》2010年第8期157-159,共3页Northern Horticulture

摘　　要：为了进一步阐明HSFA2功能,现采用Trizol法分离拟南芥叶片总RNA,用RT-PCR方法得到拟南芥HSFA2基因,经过连接、转化和蛋白诱导对其进行了原核表达研究。结果表明:从拟南芥叶片中分离到了高质量的总RNA,进而扩增到了目的基因,将其连入表达载体PGEX-KG,且转入大肠杆菌BL21中。不同温度条件下的诱导结果表明,16℃下蛋白诱导效果较好。采用这套系统,可以使AtHSFA2得以较好的表达。For further illustration of HSFA2 function, prokaryotic expression of AtHSFA2 was studied. We used Zrizol reagent to extract total RNA from Arabidopsis leaves, and cloned AtHSFA2 by RT-PCR. Throught ligation, transformation and protein-induced system, we expressed this protein in Escherichia coli BL21. The result showed that we had got AtHSFA2 by RT-PCR based on total RNA with high quanlity, sub-cloned this gene into PGEX-KG and transformed the recombinant vectors into Escherichia coli BL21. At various temperatures, it was different for protein expression and better at 16℃. As above mentioned, the system was suitable for AtHSFA2 expression.

关 键 词：热激转录因子 基因克隆 原核表达 

分 类 号：S637.903[农业科学—蔬菜学]