血皮槭的愈伤组织培养研究  

Research on the Tissue Culture of Acer griseum

作　　者：陈丽[1] 李冰[1] 王梅[1] 牛小沛 

机构地区：[1]济源市农业科学研究所,河南济源454650

出　　处：《北方园艺》2010年第8期170-172,共3页Northern Horticulture

摘　　要：对血皮槭的营养器官嫩茎、叶和叶柄进行了愈伤组织的诱导研究。结果表明:各外植体中诱导愈伤组织的难易程度为嫩茎>叶柄>叶;2,4-D比NAA更有利于愈伤组织的形成,诱导血皮槭产生愈伤组织的最佳培养基配方:改良MS+6-BA 1.8 mg/L+2,4-D 0.8 mg/L+0.6g/L活性炭,诱导率为80%;继代增殖培养基的配方:改良MS+6-BA 3.0 mg/L+2,4-D 2.0 mg/L+0.6 g/L活性炭,增殖系数可达3.0。In this experiment, various explants-tender shoots, leaves and stalk derived from the Acer griseum had been used to induce the callus. The results showed that for inducing the callus, the optimized tissue were the egments of tender shoots, secondly were the leaves stalks, finally the leaves;the optimum forum for inducing callus was improve MS＋ 6BA 1.8 mg/L＋2,4-D O. 8mg/L＋0.6 g/L charcoal,for subculture 80% ;for subculture,improving MS＋6BA 3. 0 mg/ L＋2,4-D 2. 0 mg/L＋0. 6 g/Lcharcoal could increase the propagational coefficient by 3.0.

关 键 词：血皮槭 外植体 组织培养 愈伤组织 

分 类 号：S792.35[农业科学—林木遗传育种]