Galectin-13基因在仔猪断乳前、后胃肠道组织表达差异性分析  

mRNA Expression of Galectin-13 in Piglets Gastrointestinal Tract of Pre-and Post-weaning

作　　者：孙娅莉[1] 韩立强[1] 李宏基[1] 郭豫杰[1] 鲁维飞[1] 常磊[1] 杨国宇[2] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]农业部动物生长发育调控重点开放实验室,河南郑州450002

出　　处：《华北农学报》2010年第2期56-59,共4页Acta Agriculturae Boreali-Sinica

基　　金：河南省重大科技攻关项目(0522010800)

摘　　要：从仔猪的十二指肠组织中克隆Galectin-13基因,研究Galectin-13在仔猪不同组织中的分布情况,并采用实时荧光定量PCR检测Galectin-13基因在断乳前、后仔猪胃肠道组织中的表达差异情况。结果表明:成功克隆出了猪Galectin-13基因序列,并被GenBank收录(Accession.NM_001142841)。与断乳前相比,断乳后Galectin-13基因的mR-NA在十二指肠、回肠组织中的表达量显著上调(P<0.05),而在胃、空肠、盲肠和结肠中的表达量与对照组相比差异不显著(P>0.05)。The coding sequence of galectin-13 were cloned from tissure of piglet. The gene expression of galectin- 13 were amplified by RT-PCR in various piglet tissues. The fold change of galectin-13 mRNA was detemined by Real- time PCR compared between pre-and post-weaning piglet gastrointestinal tract. The result showed that the full length of galectin-13 was 548 bp, and accepted by GenBank （Accession. NM_001142841 ）. Galectin-13 mRNA expression was intensely expressed in most tissures and was significantly raised in duodenum and ileum after weaning（P 〈0.05）.

关 键 词：猪 Galectin-13基因 组织分布 实时荧光定量PCR 表达差异 

分 类 号：Q786[生物学—分子生物学]