加拿大披碱草×老芒麦杂种加倍一代群体的聚类分析  

Cluster Analysis of C_1Ⅱ Population of E.canadensis and E. sibiricus

作　　者：李景环[1,2] 云锦凤[1] 王慈航[2] 杨敏[2] 张丽[2] 

机构地区：[1]内蒙古农业大学生态环境学院,内蒙古呼和浩特010019 [2]内蒙古师范大学生命科学与技术学院,内蒙古呼和浩特010022

出　　处：《华北农学报》2010年第2期106-111,共6页Acta Agriculturae Boreali-Sinica

基　　金："十一五"科技支撑计划项目(2008BADB3B02);内蒙古自治区重大科技项目(20071923)

摘　　要：通过EST同工酶和RAPD标记,对加拿大披碱草与老芒麦加倍一代的70个单株进行了聚类分析.结果表明,用EST标记的单株之间的相似系数在0.4和1.0之间;RAPD标记,单株之间的相似系数在0.2860和0.9918之间;加倍一代群体分为三类:分别为偏向母本加拿大披碱草的、偏向父本老芒麦的和变异较大的类群;对加倍一代群体的70个单株进行抽样研究时,应从这三个类群中按比例抽取样本,以便科学全面地对其进行研究。Cluster analysis of 70 individuals in C1Ⅱ of E. canadensisiand and E. sibiricus was done through EST and molecular markers . The results showed that the genetic similarity coefficient of 70 individuals varied from 0. 4 to 1 by EST,and the genetic similarity coefficient varied from 0. 286 0 to 0. 991 8 by RAPD. Accoding RAPD and EST markers,C1Ⅱ population can be classified into three groups：one tended to maternal E. canadensisi,the other tended to paternal E. sibiricus,and another was great variabilities. Sampling on 70 individuls in C1Ⅱ population,we can not Sam- ple at random,but proportional sampling must be used,which was beneficial to study C1Ⅱ scientifically and entirely.

关 键 词：加拿大披碱草 老芒麦 加倍一代 分子标记 聚类分析 

分 类 号：S543.9[农业科学—作物学]