农杆菌介导的番茄Micro-Tom遗传转化体系的建立  

Establishment of Agrobacterium-mediated Genetic Transformation System of Micro-Tom

作　　者：陈双臣[1] 刘爱荣[1] 王凤华[1] 王菲[1] 周洲[1] 

机构地区：[1]河南科技大学林学院,河南洛阳471003

出　　处：《华北农学报》2010年第2期112-115,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家科技支撑计划(2006BAD11A10);国家自然科学基金(30700002);河南省教育厅自然科学基金(2008B210002;2009B210004);河南科技大学校基金(2007QN008;2007QN029);河南科技大学大学生研究训练计划(SRTP)资助

摘　　要：Micro-Tom番茄植株矮小,生长密度高,生命周期短,转化效率高,成为功能基因组学研究的新型模式植物。对影响Micro-Tom遗传转化频率的共培养时间、AS的添加、工程菌液浓度和抑制农杆菌所用抗生素种类进行了分析,建立了Micro-Tom稳定高效的遗传转化体系。以bar基因设计引物对转化群体进行PCR检测,阳性率为72.3%,平均插入位点数为1.8个。遗传体系的建立为Micro-Tom在植物生物学研究中提供理论基础。Micro-Tom is a miniature dwarf tomato,which can grow at a high density, has a short life cycle, and can be transformed efficiently. As a result,it became a new model plant for functional genomics study. Co-cultivation time on regeneration, effects of AS on the regeneration frequency,Agrobacterium titer and GFP expression, antibiotic concentration selection were studied to establish stable protocol. It indicated that the average T-DNA inserted sites were 1. 8 and positive rates were 72. 3% through PCR detection.

关 键 词：Micro—Tom番茄 模式植物 根癌农杆菌 遗传转化 

分 类 号：S641.03[农业科学—蔬菜学]