小鼠IgGFc受体(moFcγRⅡ)稳定表达细胞系的建立及鉴定  

Establishment of Cell Line Stably Transfected with the Constructed Plasmid of Mus Musculus FcγRⅡ

作　　者：苗现伟[1] 席俊[1] 刘玺[2] 张改平[1] 邓瑞广[1] 李清州[1] 张利娜[1] 游雷鸣[1] 刘运超[1] 

机构地区：[1]河南省农业科学院河南省动物免疫学重点实验室,农业部动物免疫学重点开放实验室,河南郑州450002 [2]河南科技学院,河南新乡453003

出　　处：《华北农学报》2010年第2期133-135,共3页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金(30730068);国家重点基础研究发展计划(“973”计划)(2005CB523200)

摘　　要：为建立小鼠IgGFc受体(moFcγRⅡ)稳定表达的哺乳动物细胞系,PCR克隆小鼠moFcγRⅡ片段,构建真核表达载体pcDNAmoFcγRⅡ。转染COS-7细胞后通过G418抗性筛选和连续克隆,获得了在COS-7细胞表面稳定表达FcγRⅡ的真核细胞系。玫瑰花环试验结果显示其阳性率在90%以上。To establish the mouse IgGFc Receptor （moFcγRⅡ） stable expression cell line. The coding sequence of FcγRⅡ was amplified and cloned into the mammalian expression vector pcDNA3.0 under control of the CMV promoter. The COS-7 cells were tranfected with the recombinant plasmids, and the FcγRⅡ stable expression COS-7 cells were se- lected by the antibiotics of G418,and subeloned by well-known method of limiting dilution. Rosetting test of the binding of mouse IgG-sensitized chicken erythrocytes show that the percent of positive cells were more than 90%. The establish- ment of the mouse FcγRⅡ stable expression cell line laid the necessary foundation for the identification of the linear epitope of IgG binding on mouse FcγRⅡ,and further investing the immunologic mechanism of Fc receptor of IgG.

关 键 词：小鼠IgG受体(FcγRⅡ) 细胞系 玫瑰花环 稳定转染 

分 类 号：Q786[生物学—分子生物学]