三种纳米材料C_(70),C_(60)Cl_6和C_(60)Ph_6引起细胞自噬的现象  

Studies on autophagy induced by nano-C70,nano-C60Cl6 and nano-C60Ph6

作　　者：孙轲婧[1] 郑芳[1] 温龙平[1] 

机构地区：[1]中国科学技术大学生命科学学院合肥微尺度物质科学国家实验室,安徽合肥230027

出　　处：《中国科学技术大学学报》2010年第4期339-345,共7页JUSTC

基　　金：国家重点基础研究发展(973)计划(2006CB933300;2009CB930300)资助

摘　　要：为了进一步研究富勒烯家族及其衍生物引起细胞自噬的现象,运用荧光显微镜、透射电子显微镜、蛋白免疫印迹等技术,对水溶性的纳米C70,C60Cl6和C60Ph6诱导的细胞自噬现象进行了初步探讨.实验结果显示,C70,C60Cl6和C60Ph6稳定分散于水中形成的纳米颗粒,均能诱导HeLa细胞产生自噬现象,揭示了富勒烯家族及其衍生物在生物医药领域更广泛的研究和应用价值.In order to further study the autophagy induced by fullerene family and its derivatives,nano-C70,C60Cl6 and C60Ph6 were selectively investigated by means of fluorescence microscope,transmission electron microscope and western blot.The results show that,nano-C70,C60Cl6 and C60Ph6 can form crystal structures in water and induce HeLa cells into autophagy process,which indicates that fullerene family and its derivatives might play a new role in the biomedical field.

关 键 词：富勒烯 纳米颗粒 C70 C60Cl6 C60Ph6 细胞自噬 

分 类 号：Q255[生物学—细胞生物学]