麻杏石甘汤对A型流感病毒感染小鼠的免疫保护作用  

The Immune Protection of Maxingshigan Decoction on Mice Which Were Infected by A-type Influenza Virus

作　　者：李玲[1] 卢芳国[1] 熊兴耀[2] 何迎春[1] 朱应武[1] 张薇[2] 张波[1] 

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南农业大学,湖南长沙410128

出　　处：《中医药学报》2010年第2期25-28,共4页Acta Chinese Medicine and Pharmacology

基　　金：湖南省自然科学基金重点项目(项目批准号:06JJ2055);湖南省科技计划项目(2009SK3120)

摘　　要：目的:通过观察麻杏石甘汤对A型流感病毒感染小鼠免疫器官重量指数和IL-2、TNF-β蛋白表达水平的影响,探讨该方对A型流感病毒感染小鼠的免疫保护作用。方法:采用鼻腔接种法建立A型流感病毒小鼠肺适应株动物感染模型。实验设麻杏石甘汤组、利巴韦林组、抗病毒颗粒组、模型对照组和正常对照组,经灌胃给药或给生理盐水后,检测小鼠体重、肺指数、脾指数、胸腺指数和肺组织中IL-2、TNF-β的蛋白表达水平。结果:麻杏石甘汤明显提高A型流感病毒感染小鼠的体重、脾重指数、胸腺指数与肺组织中IL-2的蛋白表达水平,降低肺指数和肺组织中TNF-β的蛋白表达水平。结论:麻杏石甘汤通过提高小鼠免疫器官重量指数、调节细胞因子的蛋白表达水平而对流感病毒感染小鼠起保护作用。Objective：By observing Maxingshigan Decoction（MXSGD） on immune organ weight index of mice which were infected by A-type influenza virus and levels of IL-2、TNF-β,to explore immune protection of mice which were infected by A-type influenza virus. Methods： Use nasal inoculation to establish mouse pulmonary infection model animals by A-type influenza virus. Set up MXSGD group,ribavirin group,Anti-virus particle group,model control group and normal control group. After gavaging drug or normal saline,detection of weight in mice,lung index,spleen index,thymus index,the level of TNF-β,IL-2 in lung tissue. Result： MXSGD can obviously improve mice which were infected by A-type influenza virus：inhibited the rate of weight loss,spleen weight index,thymus index and the level of IL-2 in lung tissue by Immunohistochemical method,and reduce lung weight index and the level of TNF-β in lung tissue. Conclusion： MXSGD by enhancing mice＇s immune function,regulating a variety of inflammation-associated factor secretion levels to protect mice which were infected by A-type influenza virus.

关 键 词：麻杏石甘汤 A型流感病毒 IL-2 TNF-Β 

分 类 号：R254[医药卫生—中医内科学] R285[医药卫生—中医学]