五味子多糖对H_(22)荷瘤小鼠血清IL-2、IL-10和VEGF表达的干预作用研究  

Effects of Fructus Schizandrae Polysaccharide on the Serum IL-2,IL-10 and VEGF Expression in H_(22) Tumor-Bearing Mice

作　　者：于赫[1] 李冀[1] 王艳杰[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中医药学报》2010年第2期39-41,共3页Acta Chinese Medicine and Pharmacology

基　　金：黑龙江省博士后科学基金(LBH-Z07002)

摘　　要：目的:研究H22荷瘤小鼠血清免疫相关细胞因子IL-2、IL-10和VEGF的表达及五味子多糖的干预作用。方法:采用H22荷瘤小鼠,五味子多糖给药1周后采血,分离血清,ELISA法检测各组小鼠血清IL-2、IL-10和VEGF的表达水平。结果:H22荷瘤小鼠血清IL-2表达水平显著低于正常小鼠,VEGF的表达水平显著高于正常小鼠,五味子多糖可以上调H22荷瘤小鼠血清IL-2表达水平,但对VEGF的表达水平无明显调节作用。H22荷瘤小鼠和五味子多糖给药组小鼠血清IL-10的表达水平与正常小鼠间无显著差异。结论:似可认为五味子多糖抑制小鼠H22肝癌移植瘤的作用靶点之一是IL-2,五味子多糖通过上调血清中IL-2的表达达到抑制小鼠H22肝癌移植瘤生长的作用。Objective：To study the expression of serum immunity correlating cytokines IL-2,IL-10 and VEGF in H22 tumor-bearing mice and the intervention of fructus schizandrae polysaccharide. Methods： The blood samples were collected from the H22 tumor-bearing mice after the fructus schizandrae polysaccharide was gave them for 1 week. Then isolate serum,the level of IL-2,IL-10 and VEGF was determined by ELISA. Results： The expression level of serum IL-2 in the H22 tumor-bearing mice was significantly lower than the normal mice,while VEGF was significantly higher than the normal mice; fructus schizandrae polysaccharide could up-regulate the expression of serum IL-2 in H22 tumor-bearing mice,but had no obvious effect on the expression of VEGF. There was no significant difference of the expression level of serum IL-10 between H22 tumor-bearing mice and treatment mice with the normal mice. Conclusions： IL-2 is probably one of the targets of fructus schizandrae polysaccharide,which can inhibit the growth of mouse transplanted hepatocellular carcinoma by up-regulate the expression level of serum IL-2.

关 键 词：五味子多糖 小鼠 H22 血清 IL-2 IL-10 VEGF 

分 类 号：R285.5[医药卫生—中药学]