杧果叶绿素a/b结合蛋白基因cDNA全长克隆及其序列分析  

Cloning and characterization of the cDNA gene encoding the light harvesting chlorophyll a/b binding protein in mango

作　　者：罗聪[1] 蒋雅琴[1] 高美萍[1] 陈虎[1] 何新华[1,2] 

机构地区：[1]广西大学农学院,南宁530004 [2]广西作物遗传改良与生物技术重点实验室,南宁530007

出　　处：《果树学报》2010年第3期441-444,共4页Journal of Fruit Science

基　　金：广西自然科学基金(桂科自0542022);广西科学基金应用基础研究专项(桂科基0731040);广西大学科研基金(20090042)

摘　　要：采用RT-PCR方法克隆得了杧果叶绿素a/b结合蛋白基因(Cab)cDNA全长序列,命名为Mcab(GenBankaccession No.FJ907952)。Mcab基因全长为915bp,编码264个氨基酸,推测蛋白质分子质量为28.19ku,等电点为5.35。同源性分析表明,Mcab基因在不同植物中的一致性为72%～85%。实验分析表明,杧果Mcab基因编码的蛋白中第63～232位有一个捕光叶绿素a/b结合蛋白功能域,并且在木本植物中非常保守。亚细胞定位分析显示Mcab蛋白被定位于叶绿体,它所编码的蛋白质在绿色植物光合系统中起着重要作用。蛋白二级结构预测显示,Mcab蛋白有8个α-螺旋,5个β折叠区,21个β-转角。研究将有助于进一步了解杧果光合作用的分子机制。A full-length cDNA sequence of a homologous Cab gene was cloned by employing RT-PCR, which was named as Mcab. The accession code in GenBank is FJ907952. The Mcab is 915 bp in length, encoding a protein of 264 amino acids, with an estimated molecular weight and an isoelectric point of 28.19 ku and 5.35 respectively. A comparison of the nucleotide sequences of homologous Cab genes from different species indicated that Mcab gene had a range of 72% to 85% identity in nucleotide sequence with homologues of other plants. The results indicated that Mcab protein had one chlorophyll a/b binding domain between the 63 st to 232 st amino acid and very conservative of the position between woody plants. Protein subcellular localization prediction showed that Mcab protein was located in the chloroplast and the gene was one of important genes in photosynthesis system of green plants. Prediction of the secondary structure of the protein showed that Mcab protein had 8α helices, 5β sheets and 21β turns. The research was beneficial to the further understanding of the molecular mechanism of photosynthesis in mango.

关 键 词：杧果 叶绿素a/b结合蛋白基因 克隆 序列分析 

分 类 号：S665.1[农业科学—果树学]